{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T10:21:51Z","timestamp":1774606911602,"version":"3.50.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Brucella abortus<\/jats:italic>\n            and\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            have surface lipopolysaccharides and polysaccharides carrying\n            <jats:italic>B. melitensis<\/jats:italic>\n            -type (M) and\n            <jats:italic>B. abortus<\/jats:italic>\n            -type (A) epitopes as well as common (C) epitopes present in all smooth\n            <jats:italic>Brucella<\/jats:italic>\n            biotypes. Crude lipopolysaccharides, hydrolytic O polysaccharides, and native hapten polysaccharides of MC or AC specificity were evaluated in indirect enzyme-linked immunosorbent assays with polyclonal, monoclonal, or protein G conjugates by using sera from cattle, sheep, and goats infected with AC, MC, or AMC\n            <jats:italic>Brucella<\/jats:italic>\n            biotypes. Regardless of the antigen, the levels of antibodies were lower in goats than in sheep and highest in cattle. The diagnostic performance of the assay was not affected by the absence of lipid A-core epitopes, the presence of contaminating outer membrane proteins, the AC or MC epitopic structure of the absorbed antigen, or the conjugate used. Moreover, with sera from cattle vaccinated with\n            <jats:italic>B. abortus<\/jats:italic>\n            S19 (AC) or from sheep and goats vaccinated with\n            <jats:italic>B. melitensis<\/jats:italic>\n            Rev 1 (MC), AC and MC antigens showed similar levels of reactivity. The results show that antibodies to the C epitopes largely dominate in infection, and this is consistent with the existence of multiple overlapping C epitopes (V. Weynants, D. Gilson, A. Cloeckaert, A. Tibor, P. A. Denoel, F. Godfroid, J. N. Limet, and J.-J. Letesson, Infect. Immun. 65:1939\u20131943, 1997) rather than with one or two C epitopes. It is concluded that, by adaptation to the corresponding antibody levels, brucellosis in cattle, sheep, and goats can be diagnosed by immunosorbent assay with a single combination of conjugate and antigen.\n          <\/jats:p>","DOI":"10.1128\/cdli.5.6.749-754.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:27Z","timestamp":1577820087000},"page":"749-754","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of Lipopolysaccharides and Polysaccharides of Different Epitopic Structures in the Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis in Small Ruminants and Cattle"],"prefix":"10.1128","volume":"5","author":[{"given":"B.","family":"Alonso-Urmeneta","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a, Universidad de Navarra, Pamplona,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Mari\u0301n","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Departamento de Sanidad Animal, Servicio de Investigacio\u0301n Agraria, Diputacio\u0301n General de Arago\u0301n, Zaragoza,2 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Arago\u0301n","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a, Universidad de Navarra, Pamplona,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Blasco","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Departamento de Sanidad Animal, Servicio de Investigacio\u0301n Agraria, Diputacio\u0301n General de Arago\u0301n, Zaragoza,2 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Di\u0301az","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a, Universidad de Navarra, Pamplona,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Moriyo\u0301n","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a, Universidad de Navarra, Pamplona,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"138","article-title":"Prueba de ELISA para descartar la respuesta post-vacunal a Brucella abortus cepa 19.","volume":"8","author":"Abalos P.","year":"1993","unstructured":"Abalos P. Pinochet L. F\u00e1brega F. Prueba de ELISA para descartar la respuesta post-vacunal a Brucella abortus cepa 19. Av. Cien. Vet. 8 1993 138 143","journal-title":"Av. Cien. Vet."},{"key":"e_1_3_2_3_2","unstructured":"Alonso-Urmeneta B. C. Mar\u0131\u0301n J. M. Blasco R. D\u0131\u0301az and I. Moriy\u00f3n. Unpublished data."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.12.2642-2646.1988"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)34159-6"},{"key":"e_1_3_2_6_2","unstructured":"Alton\nG. G.\nJones\nL. M.\nAngus\nR. D.\nVerger\nJ. M.\nTechniques for the brucellosis laboratory.\n1988\nInstitut National de la Recherche Agronomique\nParis France"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.4.1070-1079.1996"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.134.16.415"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.8.1835-1840.1994"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.9.2829-2836.1989"},{"key":"e_1_3_2_11_2","first-page":"131","article-title":"Comparison of rabbit monospecific anti-Brucella antisera with mouse anti-Brucella monoclonal antibodies.","volume":"27","author":"Cherwonogrodzky J. W.","year":"1995","unstructured":"Cherwonogrodzky J. W. Comparison of rabbit monospecific anti-Brucella antisera with mouse anti-Brucella monoclonal antibodies. Arch. Med. Vet. 27 1995 131 137","journal-title":"Arch. Med. Vet."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.6.1120-1123.1988"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.12.3168-3174.1992"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08547.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.117.15.385"},{"key":"e_1_3_2_16_2","first-page":"1593","article-title":"R\u00f4le respectif en s\u00e9rologie de la brucellose bovine des antig\u00e8nes et des immunoglobulines G1 et G2 dans les tests d\u2019agglutination, de Coombs et au Rose de Bengale ainsi que dans le ph\u00e9nom\u00e8ne de zone.","volume":"274","author":"D\u0131\u0301az R.","year":"1972","unstructured":"D\u0131\u0301az R. Levieux D. R\u00f4le respectif en s\u00e9rologie de la brucellose bovine des antig\u00e8nes et des immunoglobulines G1 et G2 dans les tests d\u2019agglutination, de Coombs et au Rose de Bengale ainsi que dans le ph\u00e9nom\u00e8ne de zone. C. R. Acad. Sci. Ser. D 274 1972 1593 1596","journal-title":"C. R. Acad. Sci. Ser. D"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.96.4.893-901.1968"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.12.3136-3141.1993"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.5.1159-1165.1994"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.7.1353-1356.1988"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.5.1419-1426.1989"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.2.3.263-267.1995"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(92)90117-C"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.12.6.753-760.1980"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.5.556-564.1997"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5877(95)00513-7"},{"key":"e_1_3_2_27_2","unstructured":"Perry\nM. B.\nBundle\nD. R.\nLipopolysaccharide antigens and carbohydrates of Brucella Advances in brucellosis research.\nAdams\nL. G.\n1990\n76\n88\nTexas A&M University Press\nCollege Station"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.2.489-494.1990"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.1.2.206-213.1994"},{"key":"e_1_3_2_30_2","first-page":"513","article-title":"An hemolysis-in-gel test for bovine brucellosis.","volume":"56","author":"Ruckerbauer G. M.","year":"1984","unstructured":"Ruckerbauer G. M. Garc\u0131\u0301a M. M. Rigby C. E. Robertson F. J. Samagh B. S. Stemshorn B. W. An hemolysis-in-gel test for bovine brucellosis. Rev. Dev. Biol. Stand. 56 1984 513 520","journal-title":"Rev. Dev. Biol. Stand."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.3.3.305-308.1996"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.1.100-107.1996"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(91)90007-W"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.5.1939-1943.1997"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.10.2514-2522.1994"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.6.749-754.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.6.749-754.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:53:08Z","timestamp":1645588388000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.5.6.749-754.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,11]]}},"alternative-id":["10.1128\/CDLI.5.6.749-754.1998"],"URL":"https:\/\/doi.org\/10.1128\/cdli.5.6.749-754.1998","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,11]]},"assertion":[{"value":"1998-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-07-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}