{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:08:53Z","timestamp":1791058133376,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Genes coding for homologs of the highly conserved cell division protein FtsZ were isolated from\n            <jats:italic>Bartonella henselae<\/jats:italic>\n            and\n            <jats:italic>Bartonella quintana<\/jats:italic>\n            , the causative agents of cat scratch disease and trench fever, respectively. DNA fragments coding for the\n            <jats:italic>ftsZ<\/jats:italic>\n            open reading frames (ORFs) were cloned into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            following PCR amplification with primers based on the\n            <jats:italic>ftsZ<\/jats:italic>\n            sequence of the closely related species\n            <jats:italic>Bartonella bacilliformis<\/jats:italic>\n            . The amino acid sequences predicted from the cloned\n            <jats:italic>B. henselae<\/jats:italic>\n            and\n            <jats:italic>B. quintana ftsZ<\/jats:italic>\n            ORFs are 81 to 83% identical to the corresponding protein in\n            <jats:italic>B. bacilliformis<\/jats:italic>\n            . Like the FtsZ protein of\n            <jats:italic>B. bacilliformis<\/jats:italic>\n            , the\n            <jats:italic>B. henselae<\/jats:italic>\n            and\n            <jats:italic>B. quintana<\/jats:italic>\n            homologs are about twice as large as the FtsZ proteins reported in most other organisms. Localized sequence differences within the C-terminal coding regions of the\n            <jats:italic>Bartonella ftsZ<\/jats:italic>\n            genes were used as the basis for species-specific identification of these organisms at both the DNA and protein levels. Oligonucleotide primers which permit the amplification of an\n            <jats:italic>ftsZ<\/jats:italic>\n            fragment from each of the\n            <jats:italic>Bartonella<\/jats:italic>\n            species without amplifying DNA from the other two species were designed. Anti-FtsZ antisera raised in rabbits against synthetic peptides corresponding to the relatively divergent C-terminal regions were shown via Western blot analysis to react only with the FtsZ protein from the cognate\n            <jats:italic>Bartonella<\/jats:italic>\n            species. These observations raise the possibility that the differences in\n            <jats:italic>ftsZ<\/jats:italic>\n            sequences can be used as the basis for diagnostic tests to differentiate among these closely related pathogens.\n          <\/jats:p>","DOI":"10.1128\/cdli.5.6.766-772.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:30Z","timestamp":1577820090000},"page":"766-772","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Use of the Cell Division Protein FtsZ as a Means of Differentiating among\n            <i>Bartonella<\/i>\n            Species"],"prefix":"10.1128","volume":"5","author":[{"given":"Timothy M.","family":"Kelly","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Georgia State University, Atlanta, Georgia 30302,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indira","family":"Padmalayam","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara R.","family":"Baumstark","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Georgia State University, Atlanta, Georgia 30302,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00402.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.4.942-948.1994"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1931-1937.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.8.1952-1956.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.1.1-7.1987"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.19.6145-6151.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1995.00430150164017"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)90012-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1997.tb123245.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.42.215"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/172.2.570"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.173"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.9.2270-2274.1996"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01701.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.21.6788-6797.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.18.5822-5830.1991"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01708232"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2283-2287.1997"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.6.2644-2648.1994"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.9.2754-2758.1994"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1797-1803.1995"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.14.4545-4552.1997"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0101.950103"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92032-B"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199012063232301"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1089-1093.1995"},{"key":"e_1_3_2_29_2","unstructured":"Sambrook\nJ.\nFritsch\nE. 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