{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T05:22:28Z","timestamp":1722921748314},"reference-count":20,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A western blot (WB) test was evaluated for detection of antibodies against native glycosylated and chemically deglycosylated M and H antigens of\n            <jats:italic>Histoplasma capsulatum<\/jats:italic>\n            in serum obtained from patients during the acute phase of pulmonary histoplasmosis that occurred during an outbreak. Of 275 serum samples tested by immunodiffusion and complement fixation (CF) samples from 40 patients affected during this outbreak and from 37 negative controls were tested by WB test. A group of patients whose sera were negative for CF antibodies and precipitins early in the acute stage of histoplasmosis but who all seroconverted during convalescence 6 weeks later were tested with the WB test. Antibodies against untreated H and M antigens were detected at a 1:100 dilution by WB test in 45% of the 20 acute-phase serum samples and in all 20 of the convalescent-phase specimens. The WB test\u2019s sensitivity for acute-phase specimens increased to 90% (18 of 20 specimens) when H and M antigens were treated by periodate oxidation to inactivate susceptible carbohydrate epitopes. When native glycosylated antigens were used in the WB test, positive reactions were observed in negative control serum specimens (3 of 37 specimens; 8%) and in serum specimens obtained from asymptomatic persons screened as part of the outbreak investigation (13 of 20 specimens; 65%). These positive reactions were also attributed to glycosidic epitopes since the specificity of the WB test increased from 78 to 100% when periodate-treated H and M antigens were used. WB test with deglycosylated H and M antigens of histoplasmin provides a rapid, sensitive, and specific test to diagnose acute pulmonary histoplasmosis before precipitins can be detected.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.1.20-23.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:49Z","timestamp":1577820109000},"page":"20-23","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of a Western Blot Test in an Outbreak of Acute Pulmonary Histoplasmosis"],"prefix":"10.1128","volume":"6","author":[{"given":"Claudia V.","family":"Pizzini","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Micologia Medica, Hospital Evandro Chagas, Fundac\u0327ao Oswaldo Cruz,1and"},{"name":"<!--label omitted: 2-->Instituto de Microbiologia, Universidade Federal do Rio de Janeiro,2 Rio de Janeiro, Brazil, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosely M.","family":"Zancope\u0301-Oliveira","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Micologia Medica, Hospital Evandro Chagas, Fundac\u0327ao Oswaldo Cruz,1and"},{"name":"<!--label omitted: 2-->Instituto de Microbiologia, Universidade Federal do Rio de Janeiro,2 Rio de Janeiro, Brazil, and"},{"name":"<!--label omitted: 3-->Centers for Disease Control and Prevention,3 Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Errol","family":"Reiss","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Centers for Disease Control and Prevention,3 Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rana","family":"Hajjeh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Centers for Disease Control and Prevention,3 Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo","family":"Kaufman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Centers for Disease Control and Prevention,3 Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301 Mauro","family":"Peralta","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Instituto de Microbiologia, Universidade Federal do Rio de Janeiro,2 Rio de Janeiro, Brazil, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01576782"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(73)90028-4"},{"key":"e_1_3_1_4_2","first-page":"9","article-title":"Serodiagnosis of histoplasmosis","volume":"1","author":"Davies S. F.","year":"1986","unstructured":"Davies S. F. Serodiagnosis of histoplasmosis Seminars in respiratory infections Sarosi G. A. 1 1986 9 15 Grune & Stratton Orlando, Fla","journal-title":"Seminars in respiratory infections"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(18)30596-5"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2252-2255.1997"},{"key":"e_1_3_1_7_2","unstructured":"Hajjeh\nR.\nTurf\nE.\nWoolard\nD.\nKaufman\nL.\nPadhye\nA.\nMcNeil\nM.\nAn outbreak of acute histoplasmosis (AH) in a correctional center in Virginia abstr. K201\nAbstracts of the 35th General Meeting of the Interscience Conference on Antimicrobial Agents and Chemotherapy 1995.\n1995\n324\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.Supplement_1.S23"},{"key":"e_1_3_1_9_2","unstructured":"Kaufman\nL.\nKovacs\nJ. A.\nReiss\nE.\nClinical immunomycology\nManual of clinical laboratory immunology\n5th ed.\nRose\nN. R.\nde Macario\nE. C.\nFolds\nJ. D.\nLane\nH. C.\nNakamura\nR. M.\n1997\n575\n584\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.48.3.806-812.1985"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.53.3.540-546.1986"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(83)92032-3"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(79)91168-9"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-97-5-680"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198601093140205"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01964063"},{"key":"e_1_3_1_17_2","first-page":"7","article-title":"Histoplasmosis in the acquired immunodeficiency syndrome.","volume":"7","author":"Wheat L. J.","year":"1996","unstructured":"Wheat L. J. Histoplasmosis in the acquired immunodeficiency syndrome. Curr. Top. Med. Mycol. 7 1996 7 18","journal-title":"Curr. Top. Med. Mycol."},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219380000041"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.1.5.563-568.1994"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.1.4.390-393.1994"},{"key":"e_1_3_1_21_2","unstructured":"Zancop\u00e9-Oliveira\nR. M.\nMayer\nL. W.\nReiss\nE.\nLott\nT. J.\nIsolation and characterization of the M antigen of Histoplasma capsulatum abstr. F-35\nAbstracts of the 97th General Meeting of the American Society for Microbiology Washington D.C\n1997\n266"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.6.1.20-23.1999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.6.1.20-23.1999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:51:57Z","timestamp":1645588317000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.6.1.20-23.1999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999,1]]}},"alternative-id":["10.1128\/CDLI.6.1.20-23.1999"],"URL":"https:\/\/doi.org\/10.1128\/cdli.6.1.20-23.1999","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,1]]},"assertion":[{"value":"1998-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-10-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}