{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:56:57Z","timestamp":1791053817721,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Mycosis fungoides (MF) and Sezary syndrome (SS) are the two main clinical entities of cutaneous T-cell lymphoma (CTCL). As the disease progresses from MF to SS, a switch from a type 1 (interleukin [IL]-2 and gamma interferon [IFN-\u03b3]) to a type 2 (IL-4) cytokine production profile occurs. Although roles for type 1 and type 2 cytokines in the pathogenesis of CTCL have been proposed, the cellular origins of these cytokines are unclear. Using flow cytometry to identify individual T-cell subsets, we studied cytokine synthesis by the T cells of 13 patients with SS and 12 with MF and 9 hematologically healthy donors. Upon activation with phorbol 12-myristate 13-acetate (PMA), the numbers of T cells synthesizing IL-2 were similar for all study groups. Whereas the predominant T-cell producing IL-2 in healthy donors and in those with MF was CD7<jats:sup>+<\/jats:sup>, in patients with SS, it was CD7<jats:sup>\u2212<\/jats:sup>. Although the number of IL-4<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>T cells was low for all study groups, there was a significantly higher number of IL-4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>T cells in patients with MF than in those with SS or healthy donors. There was a decline in the number of IFN-\u03b3-producing T cells in CTCL donors compared to that in healthy donors. More importantly, there was a significant decrease in the number of IFN-\u03b3-producing T cells with disease progression from MF to SS. The inability of these T cells to synthesize IFN-\u03b3 may have prognostic value in CTCL, since it may be responsible for the progression of the disease from MF to SS.<\/jats:p>","DOI":"10.1128\/cdli.6.1.79-84.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:55Z","timestamp":1577820115000},"page":"79-84","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Dysregulated Synthesis of Intracellular Type 1 and Type 2 Cytokines by T Cells of Patients with Cutaneous T-Cell Lymphoma"],"prefix":"10.1128","volume":"6","author":[{"given":"Bang-Ning","family":"Lee","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Pathology and Laboratory Medicine1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Duvic","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Section of Dermatology2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Kwang","family":"Tang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Pathology and Laboratory Medicine1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Bueso-Ramos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Pathology and Laboratory Medicine1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeev","family":"Estrov","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Bioimmunotherapy,3 Division of Medicine, The University of Texas M. 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