{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T16:06:18Z","timestamp":1760889978929},"reference-count":37,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1999,5,1]],"date-time":"1999-05-01T00:00:00Z","timestamp":925516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In a search for new anti-autoimmune agents that selectively suppress activation of autoreactive T cells, one such agent, 5-methyl-3-(1-methylethoxy)benzo[b]thiophene-2-carboxamide (CI-959-A), was found to be effective. This compound, which is known to suppress tumor necrosis factor alpha (TNF-\u03b1)-induced CD54 expression, inhibited the primary proliferative response of the T cell to antigen (Ag)-presenting cells (APCs) including allogenic dendritic cells (DCs), autologous Epstein-Barr virus-infected B cells, and human T lymphotropic virus type I (HTLV-I)-infected T cells. Autoreactive T cells from patients with HTLV-I-associated myelopathy\/tropical spastic paraparesis (HAM\/TSP) spontaneously proliferate in vitro, and their activation is reported to be associated with CD54 expression. The spontaneous proliferation of T cells from patients with HAM\/TSP was entirely blocked by CI-959-A. However, in this study, the T-cell proliferation in 15 patients with HAM\/TSP was found to depend more extensively on major histocompatibility complex (MHC) class II and CD86 than on CD54 Ags. Since most important APCs for the development of HAM\/TSP are DCs and HTLV-I-infected T cells, the effect of CI-959-A on DC generation and on the expression of surface molecules on activated T cells is examined. CI-959-A suppressed recombinant granulocyte-macrophage colony stimulating factor (GM-CSF)- and recombinant interleukin-4-dependent differentiation of DCs from monocytes and inhibited the expression of CD54 and, more extensively, MHC class II and CD86 Ags. CI-959-A showed little toxicity toward lymphoma or HTLV-I-infected T-cell lines or toward monocytes and cultured DCs. These results suggest that CI-959-A might be a potent anti-HAM\/TSP agent.<\/jats:p>","DOI":"10.1128\/cdli.6.3.316-322.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:22:29Z","timestamp":1577820149000},"page":"316-322","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Marked Suppression of T Cells by a Benzothiophene Derivative in Patients with Human T-Lymphotropic Virus Type I-Associated Myelopathy\/Tropical Spastic Paraparesis"],"prefix":"10.1128","volume":"6","author":[{"given":"Masahiko","family":"Makino","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Human Retroviruses1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyuki","family":"Azuma","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Allergy and Immunology, National Children\u2019s Medical Research Center, Setagaya-ku, Tokyo,2Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-Ichi","family":"Wakamatsu","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Human Retroviruses1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Suruga","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Human Retroviruses1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuji","family":"Izumo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Division of Molecular Pathology,3 Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchel M.","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Human Retroviruses1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Baba","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Human Retroviruses1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/366076a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(96)00079-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm00032a001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6110"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3525","DOI":"10.4049\/jimmunol.154.7.3525","article-title":"Induction of persistent T cell hyporesponsiveness in vivo by monoclonal antibody to ICAM-1 in patients with rheumatoid arthritis.","volume":"154","author":"Davis L. 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