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This was increased in a dose-dependent manner in the control groups consisting of healthy blood donors and patients with pulmonary tuberculosis. In contrast, PMNLs from the HIV and HIV\/TB groups responded reciprocally in the same assay; that is, higher IL-8 input concentrations resulted in the release of less enzyme than lower IL-8 input concentrations. The degranulation response of PMNLs from HIV-1-infected individuals was similarly altered for another agonist,<jats:italic>N<\/jats:italic>-formyl-methionyl-leucyl-phenylalanine, suggesting that impairment of the nonoxidative armature of PMNL was a more generalized phenomenon. However, impaired IL-8-induced degranulation was found to be associated with the reduced expression of both IL-8 receptors, A and B, on whole-blood PMNLs from HIV-1-infected patients compared with that on whole-blood PMNLs from healthy persons. The density of IL-8RA, in particular, was most reduced on the surfaces of PMNLs from those patients with the poorest degranulation in response to IL-8. Inefficient agonist-induced degranulation may contribute to the increased susceptibility of HIV-1-infected persons to secondary microbial infections, this being further exacerbated in HIV\/TB patients who, in addition, display defects in phagocytosis and oxidative burst.<\/jats:p>","DOI":"10.1128\/cdli.6.3.345-351.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:22:31Z","timestamp":1577820151000},"page":"345-351","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Impaired Interleukin-8-Induced Degranulation of Polymorphonuclear Neutrophils from Human Immunodeficiency Virus Type 1-Infected Individuals"],"prefix":"10.1128","volume":"6","author":[{"given":"Stephen","family":"Meddows-Taylor","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->MRC AIDS Virus Research Unit, National Institute for Virology, and Department of Virology, University of the Witwatersrand, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Desmond J.","family":"Martin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->MRC AIDS Virus Research Unit, National Institute for Virology, and Department of Virology, University of the Witwatersrand, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline T.","family":"Tiemessen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->MRC AIDS Virus Research Unit, National Institute for Virology, and Department of Virology, University of the Witwatersrand, Johannesburg, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199310000-00032"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"5682","DOI":"10.4049\/jimmunol.153.12.5682","article-title":"Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes.","volume":"153","author":"Chuntharapai A.","year":"1994","unstructured":"Chuntharapai A. 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