{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:07:02Z","timestamp":1793048822887,"version":"4.5.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1999,5,1]],"date-time":"1999-05-01T00:00:00Z","timestamp":925516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To test whether antibiotic therapy hampers the antibody response to\n            <jats:italic>Brucella<\/jats:italic>\n            antigens, 30 BALB\/c mice were infected with\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            H38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups DOX7 and DOX28, respectively) or for no treatment (control group). Antibodies to smooth lipopolysaccharide (LPS) reached peak levels (mean optical density [OD] = 2.618) between days 56 and 70 p.i. in the control group, and similar peak levels (mean OD = 2.486) were observed in the DOX28 group, but significantly lower peak levels (mean OD = 0.821) were observed at 28 days p.i. in the DOX7 group. The antibody response against cytoplasmic proteins depleted of LPS (CPs) reached maximal levels (mean OD = 2.402) between days 56 and 70 p.i. in the control group, but no response was detected in the DOX7 group. Anti-CP antibodies were detected in only three animals from the DOX28 group, at levels significantly lower than those in the control group (mean maximal OD = 0.791). The pattern of antibody response to an 18-kDa cytoplasmic protein of\n            <jats:italic>Brucella<\/jats:italic>\n            spp. was similar to that against the CP antigen. This study shows that early antibiotic treatment affects the antibody response of mice to cytoplasmic proteins of\n            <jats:italic>Brucella<\/jats:italic>\n            and, to a lesser extent, to LPS.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.3.440-443.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:22:41Z","timestamp":1577820161000},"page":"440-443","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Effect of Early Antibiotic Treatment on the Antibody Response to Cytoplasmic Proteins of\n            <i>Brucella melitensis<\/i>\n            in Mice"],"prefix":"10.1128","volume":"6","author":[{"given":"Raul A.","family":"Bowden","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Inmunoqu\u0131\u0301mica y Biotecnolog\u0131\u0301a, Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, UNICEN, 7000 Tandil,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela C.","family":"Racaro","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->IDEHU, Facultad de Farmacia y Bioqu\u0131\u0301mica, Universidad de Buenos Aires, 1113 Buenos Aires,2 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo C.","family":"Baldi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->IDEHU, Facultad de Farmacia y Bioqu\u0131\u0301mica, Universidad de Buenos Aires, 1113 Buenos Aires,2 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.8.1909-1912.1989"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.5.1241"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.3.446"},{"key":"e_1_3_2_5_2","unstructured":"Baldi P. C. G. H. Giambartolomei J. C. Wallach C. A. Velikovsky and C. A. Fossati. Different patterns of antibody response to lipopolysaccharide and cytoplasmic proteins of Brucella in human brucellosis of recent onset. Submitted for publication."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-45-3-206"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(96)01265-5"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1995.tb00180.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.2.219"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.3.604-607.1992"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.8.2141-2145.1993"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.2.632-638.1994"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.5.556-564.1997"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(96)00018-7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.5.918"},{"key":"e_1_3_2_16_2","unstructured":"Sande\nM. A.\nMandell\nG. L.\nAgentes antimicrobianos: tetraciclinas cloramfenicol eritromicina y agentes antibacterianos varios\nLas bases farmacol\u00f3gicas de la terap\u00e9utica.\nGoodman Gilman\nA.\nRall\nT. W.\nNies\nA. S.\nTaylor\nP.\n1991\n1083\n1109\nEditorial M\u00e9dica Panamericana\nBuenos Aires Argentina"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","unstructured":"Serre\nA.\nImmunology and pathophysiology of human brucellosis\nBrucellosis: clinical and laboratory aspects.\nYoung\nE. J.\nCorbel\nM. J.\n1989\n85\n95\nCRC Press\nBoca Raton Fla","DOI":"10.1201\/9781003068518-9"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.6.3.440-443.1999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.6.3.440-443.1999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:05:49Z","timestamp":1645589149000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.6.3.440-443.1999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,5]]}},"alternative-id":["10.1128\/CDLI.6.3.440-443.1999"],"URL":"https:\/\/doi.org\/10.1128\/cdli.6.3.440-443.1999","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,5]]},"assertion":[{"value":"1998-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-02-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}