{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:12:32Z","timestamp":1789873952009,"version":"4.0.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>Taenia solium<\/jats:italic>\n            metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione\n            <jats:italic>S<\/jats:italic>\n            -transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Re\u0301union and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.4.479-482.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:22:46Z","timestamp":1577820166000},"page":"479-482","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Serological Diagnosis of Human Cysticercosis by Use of Recombinant Antigens from\n            <i>Taenia solium<\/i>\n            Cysticerci"],"prefix":"10.1128","volume":"6","author":[{"given":"Kerstin","family":"Hubert","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Hygiene und Mikrobiologie, Universita\u0308t Wu\u0308rzburg, 97080 Wu\u0308rzburg, Germany1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abel","family":"Andriantsimahavandy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Parasitology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar2; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Michault","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Parasitology, Regional Hospital of Saint-Pierre, La Re\u0301union3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Frosch","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Hygiene und Mikrobiologie, Universita\u0308t Wu\u0308rzburg, 97080 Wu\u0308rzburg, Germany1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fritz A.","family":"Mu\u0308hlschlegel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Hygiene und Mikrobiologie, Universita\u0308t Wu\u0308rzburg, 97080 Wu\u0308rzburg, Germany1;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1997.d01-379.x"},{"key":"e_1_3_2_3_2","unstructured":"Andriantsimahavandy\nA.\nMichelaut\nA.\nAndrianirina\nW.\nRakotondrazaka\nJ.\nRabarijaona\nL.\nBoisier\nP.\nChanteau\nS.\nEvaluation d\u2019un antig\u00e8ne glycoprot\u00e9ique purifi\u00e9 pour le diagnostic de la cysticercose: application \u00e0 la s\u00e9ro\u00e9pid\u00e9miologie.\n4\u00e8mes Actualit\u00e9s du Pharo et de l\u2019Hopital Laveran Marseille France\n1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90097-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019052-199706000-00017"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(96)00130-X"},{"key":"e_1_3_2_7_2","first-page":"324","article-title":"Preparation and sequence analysis of Taenia crassiceps metacestode recombinant antigens with potential for immunodiagnosis of human cerebral cysticercosis.","volume":"45","author":"Fischer C.","year":"1994","unstructured":"Fischer C. 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