{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T20:02:32Z","timestamp":1700078552074},"reference-count":29,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An enzyme-linked immunoelectrotransfer blot for the diagnosis of human hydatid disease was performed, and the different antibody responses were analyzed by a discriminant analysis. This multivariate technique gave us, first, a selection of the most important responses against\n            <jats:italic>Echinococcus granulosus<\/jats:italic>\n            infection and, second, a procedure for the classification of patients into two groups: patients with hydatid disease and patients without a history of hydatid disease. This method was applied to 67 patients, 25 with active hydatid cysts (24 hepatic and 1 pulmonary) and 42 without a history of hydatid disease and was compared with the results obtained by conventional serology: indirect hemagglutination, latex particle agglutination, and basophil degranulation. An immunoelectrotransfer blot coupled to a discriminant analysis was more sensitive than conventional serological diagnosis and detected 100% of patients with an active hepatic hydatid cyst with a specificity of 100%. This method, however, failed to detect an uncomplicated hyaline pulmonary hydatid cyst.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.4.504-508.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:22:52Z","timestamp":1577820172000},"page":"504-508","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Immunological Diagnosis of Human Cystic Echinococcosis: Utility of Discriminant Analysis Applied to the Enzyme-Linked Immunoelectrotransfer Blot"],"prefix":"10.1128","volume":"6","author":[{"given":"I.","family":"Gadea","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Ayala","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Statistics and Operation Research,2 University of Valencia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. T.","family":"Diago","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Image Diagnosis,3 Clinic Hospital of Valencia, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Cun\u0303at","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Image Diagnosis,3 Clinic Hospital of Valencia, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Garci\u0301a","family":"de Lomas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Anderson\nT. 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