{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:28:02Z","timestamp":1761017282783},"reference-count":12,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Dengue has become hyperendemic in many islands of the Caribbean region. The performance in a diagnostic laboratory of four commercial assays for detection of immunoglobulin M (IgM) antibodies was evaluated. Sera from 62 patients with dengue virus infection were studied. These included 18 patients from whom dengue virus type 2 was isolated in a 1997 outbreak (specimens collected a mean of 14 days after onset of symptoms), 8 patients with dengue hemorrhagic fever (mean time after onset, 11 days), and 36 patients in whom dengue was previously confirmed by serology (mean time after onset, 10 days). Thirty serum specimens from blood donors in a country where dengue is not endemic were used as negative controls. The methods evaluated were two IgM-capture enzyme-linked immunosorbent assays (ELISA) (MRL Diagnostics, Cypress, Calif., and PanBio, Queensland, Australia), a dot ELISA dipstick assay (Integrated Diagnostics, Baltimore, Md.), and a rapid immunochromatographic assay for dengue IgG and IgM (PanBio IC). IgG antibodies were also detected by an ELISA method (MRL Diagnostics). The sensitivities of the four assays were as follows: MRL Diagnostics IgM ELISA, 98.4%; PanBio IgM ELISA, 85.5%; Integrated Diagnostics IgM dot ELISA, 96.8%; and PanBio IC, 83.9%. The specificities of all tests were 100%. Evidence of secondary dengue was found in all patients with dengue hemorrhagic fever and in 83% of the remaining patients. The MRL Diagnostics IgM ELISA appears to be more sensitive than the PanBio IgM ELISA, and this may be significant when IgM titers are low, particularly in patients with secondary dengue infections. The dot ELISA dipstick assay is equally sensitive and may be more appropriate for use in laboratories with lower workloads.<\/jats:p>","DOI":"10.1128\/cdli.6.4.555-557.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:23:01Z","timestamp":1577820181000},"page":"555-557","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of Four Methods for Detection of Immunoglobulin M Antibodies to Dengue Virus"],"prefix":"10.1128","volume":"6","author":[{"given":"Songee L.","family":"Branch","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Leptospira Laboratory, Ministry of Health &amp; the Environment,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul N.","family":"Levett","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Leptospira Laboratory, Ministry of Health &amp; the Environment,1 and"},{"name":"<!--label omitted: 2-->University of the West Indies, School of Clinical Medicine &amp; Research,2 Barbados"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.480"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","unstructured":"Halstead\nS. B.\nEmergence mechanisms in yellow fever and dengue\nEmerging infections 2.\nScheld\nW. M.\nCraig\nW. A.\nHughes\nJ. M.\n1998\n65\n79\nAmerican Society for Microbiology\nWashington D.C","DOI":"10.1128\/9781555816957.ch3"},{"key":"e_1_3_2_4_2","first-page":"161","article-title":"A point source dengue outbreak in Canadian tourists in Barbados.","volume":"24","author":"Laboratory Centre for Disease Control","year":"1998","unstructured":"Laboratory Centre for Disease Control A point source dengue outbreak in Canadian tourists in Barbados. Can. Commun. Dis. Rep. 24 1998 161 164","journal-title":"Can. Commun. Dis. Rep."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0197(98)00002-6"},{"key":"e_1_3_2_6_2","first-page":"1","article-title":"Re-emergence of dengue in the Americas.","volume":"18","author":"Pan American Health Organization","year":"1997","unstructured":"Pan American Health Organization Re-emergence of dengue in the Americas. Epidemiol. Bull. 18 1997 1 6","journal-title":"Epidemiol. Bull."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.1.7-10.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.3.407-409.1998"},{"key":"e_1_3_2_9_2","first-page":"235","article-title":"Don\u2019t forget dengue! Clinical features of dengue fever in returning travellers.","volume":"32","author":"Shirtcliffe P.","year":"1998","unstructured":"Shirtcliffe P. Cameron E. Nicholson K. G. Wiselka M. J. Don\u2019t forget dengue! Clinical features of dengue fever in returning travellers. J. R. Coll. Phys. London 32 1998 235 237","journal-title":"J. R. Coll. Phys. London"},{"key":"e_1_3_2_10_2","first-page":"38","article-title":"DHF\/DSS: an unwelcome arrival in Trinidad.","volume":"46","author":"Teelucksingh S.","year":"1997","unstructured":"Teelucksingh S. Mangray A. S. Barrow S. Jankey N. Prabhakar P. Lewis M. DHF\/DSS: an unwelcome arrival in Trinidad. West Indian Med. J. 46 1997 38 42","journal-title":"West Indian Med. J."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.234-238.1998"},{"key":"e_1_3_2_12_2","first-page":"145","article-title":"Aspects de la dengue a la Martinique en 1995\u20131996.","volume":"58","author":"Villeneuve L.","year":"1998","unstructured":"Villeneuve L. Mansuy J. M. Magnaval J. F. Schlegel L. Aspects de la dengue a la Martinique en 1995\u20131996. Med. Trop. 58 1998 145 148","journal-title":"Med. Trop."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.4.452-457.1997"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.6.4.555-557.1999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.6.4.555-557.1999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:09:49Z","timestamp":1645589389000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.6.4.555-557.1999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1999,7]]}},"alternative-id":["10.1128\/CDLI.6.4.555-557.1999"],"URL":"https:\/\/doi.org\/10.1128\/cdli.6.4.555-557.1999","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,7]]},"assertion":[{"value":"1998-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-02-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}