{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:27:57Z","timestamp":1767713277526},"reference-count":59,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The objective of the present study was to investigate the usefulness of a recombinant flagellar protein, FlaB, of\n            <jats:italic>Leptospira interrogans<\/jats:italic>\n            serovar\n            <jats:italic>pomona<\/jats:italic>\n            in the serodiagnosis of leptospirosis by the fluorescence polarization assay (FPA). The recombinant protein FlaB was purified to homogeneity by a combination of nickel-nitriloacetic acid agarose chromatography, electrophoresis, and electroelution. Purified FlaB was labeled with fluorescein isothiocyanate (FITC). Western blotting was performed by using bovine sera with microscopic agglutination test (MAT) titers of antibodies against\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovar\n            <jats:italic>pomona<\/jats:italic>\n            and\n            <jats:italic>L. bergpetersenii<\/jats:italic>\n            serovars\n            <jats:italic>hardjo<\/jats:italic>\n            and\n            <jats:italic>sejroe<\/jats:italic>\n            to confirm the antigenicity of FlaB. Western blot analysis demonstrated that labeled as well as unlabeled FlaB was recognized by the positive sera tested, indicating the broad serovar cross-reactivity of this protein. It also indicated that labeling with FITC did not affect the antigenicity. By using FITC-labeled FlaB as a tracer antigen, a homogeneous FPA was developed to detect antileptospiral antibodies in bovine sera. A population of 208 MAT-positive and 208 MAT-negative serum samples was tested by FPA. The FPA cutoff was determined by receiver operating characteristic analysis. By FPA, 83.7% of the MAT-positive serum samples were positive and 81.2% of the MAT-negative serum samples were negative. Compared to the results of MAT, the positive predictive value of FPA was 81.7% and the negative predictive value of FPA was 83.3%. The FPA is a simple and rapid technique for the detection of anti-\n            <jats:italic>Leptospira<\/jats:italic>\n            antibodies.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.4.599-605.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:23:06Z","timestamp":1577820186000},"page":"599-605","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Use of Recombinant Flagellin Protein as a Tracer Antigen in a Fluorescence Polarization Assay for Diagnosis of Leptospirosis"],"prefix":"10.1128","volume":"6","author":[{"given":"Nasreen I.","family":"Bughio","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Lin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Om P.","family":"Surujballi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(96)00729-7"},{"key":"e_1_3_2_3_2","first-page":"387","article-title":"Antibodies against leptospiral axial filament in human anti-Leptospira sera.","volume":"11","author":"Adler B.","year":"1979","unstructured":"Adler B. 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