{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T16:14:55Z","timestamp":1779293695810,"version":"3.51.4"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1999,9,1]],"date-time":"1999-09-01T00:00:00Z","timestamp":936144000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In Great Britain a recent independent scientific review for the government has concluded that the development of a cattle vaccine against<jats:italic>Mycobacterium bovis<\/jats:italic>holds the best long-term prospect for tuberculosis control in British herds. A sine qua non for vaccination is the development of a complementary diagnostic test to differentiate between vaccinated animals and those infected with<jats:italic>M. bovis<\/jats:italic>so that test-and-slaughter-based control strategies can continue alongside vaccination. In order to assess the feasibility of developing a differential diagnostic test for a live vaccine, we chose<jats:italic>M. bovis<\/jats:italic>BCG Pasteur as a model system. Recombinant forms of antigens which are expressed in<jats:italic>M. bovis<\/jats:italic>but not, or only at low levels, in BCG Pasteur (ESAT-6, MPB64, MPB70, and MPB83) were produced. These reagents were tested either alone or in combination by using peripheral blood mononuclear cells from<jats:italic>M. bovis<\/jats:italic>-infected, BCG-vaccinated, and<jats:italic>Mycobacterium avium<\/jats:italic>-sensitized calves. All four antigens induced in vitro proliferation and gamma interferon responses only in<jats:italic>M. bovis<\/jats:italic>-infected animals. A cocktail composed of ESAT-6, MPB64, and MPB83 identified infected animals but not those vaccinated with BCG. In addition, promiscuous T-cell epitopes of ESAT-6, MPB64, and MPB83 were formulated into a peptide cocktail. In T-cell assays with this peptide cocktail, infected animals were identified with frequencies similar to those obtained in assays with the protein cocktail, while BCG-vaccinated or<jats:italic>M. avium<\/jats:italic>-sensitized animals did not respond. In summary, our results suggest that peptide and protein cocktails can be designed to discriminate between<jats:italic>M. bovis<\/jats:italic>infection and BCG vaccination.<\/jats:p>","DOI":"10.1128\/cdli.6.5.675-682.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:23:15Z","timestamp":1577820195000},"page":"675-682","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Development of Diagnostic Reagents To Differentiate between<i>Mycobacterium bovis<\/i>BCG Vaccination and<i>M. bovis<\/i>Infection in Cattle"],"prefix":"10.1128","volume":"6","author":[{"given":"H. M.","family":"Vordermeier","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->TB Research Group,1and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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