{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T23:28:07Z","timestamp":1700522887613},"reference-count":12,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1999,9,1]],"date-time":"1999-09-01T00:00:00Z","timestamp":936144000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and animal sera. This assay has two advantages over a conventional indirect ELISA: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. The VT1 (Shiga-like toxin 1) antibody-positive rates were 5% in 202 normal adult humans and 99% in 93 normal cattle at a dairy farm. This ELISA is most suitable for seroepidemiologic studies of infections with VT-producing\n            <jats:italic>Escherichia coli<\/jats:italic>\n            in humans and various animal species.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.5.701-704.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:23:19Z","timestamp":1577820199000},"page":"701-704","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["A Reverse-Sandwich Enzyme-Linked Immunosorbent Assay for Verocytotoxin 1 and 2 Antibodies in Human and Bovine Sera"],"prefix":"10.1128","volume":"6","author":[{"given":"H.","family":"Miyazawa","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Technology, Kyorin University School of Health Sciences, Hachioji-shi,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Bannai","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Technology, Kyorin University School of Health Sciences, Hachioji-shi,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Yanase","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu-shi, Hokkaido,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Morita","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu-shi, Hokkaido,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Satoh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Denka Seiken Co., 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