{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T09:51:45Z","timestamp":1695635505667},"reference-count":17,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,11]]},"abstract":"ABSTRACT<\/jats:title>A gastrointestinal explant culture system was developed and compared to the mononuclear cell extraction and enzyme-linked immunospot assay method for measurement of immunoglobulin A (IgA) and IgG antibody-secreting cells (ASCs) in gastric antral and duodenal biopsies of non-Helicobacter pylori<\/jats:italic>-infected volunteers. IgA and IgG were detected in explant supernatants during 6 to 7 days of culture in all subjects. IgA containing secretory component was also detected throughout the culture period, although peak production occurred only in the first 3 days. During 7 days of culture, the cumulative geometric mean IgA levels produced were 2.2 and 8.02 \u03bcg\/ml\/10 mg of antral and duodenal biopsy tissues, respectively, while the cumulative geometric mean IgG levels were 1.54 and 2.92 \u03bcg\/ml\/10 mg of antral and duodenal biopsy tissues, respectively. Cycloheximide treatment resulted in a >90% reduction in both immunoglobulin classes after 6 days of treatment compared to levels in untreated controls. The detection of IgA and IgG ASCs extracted from biopsies on days 1 and 6 of culture confirmed that the antibody detected was derived from mucosal lamina propria. The IgA and IgG ASC responses were positively correlated with antibody concentrations detected in culture supernatants (r<\/jats:italic>= 0.87 and 0.85, respectively). These results validate the potential usefulness of our gastrointestinal explant system for the evaluation of mucosal effector B-cell function.<\/jats:p>","DOI":"10.1128\/cdli.6.6.803-807.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:23:39Z","timestamp":1577820219000},"page":"803-807","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Validation of a Gastrointestinal Explant System for Measurement of Mucosal Antibody Production"],"prefix":"10.1128","volume":"6","author":[{"given":"Genevieve A.","family":"Losonsky","sequence":"first","affiliation":[{"name":"Center for Vaccine Development, Division of Infectious Diseases and Tropical Pediatrics and Division of Geographic Medicine,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George T.","family":"Fantry","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Department of Medicine,2University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mardi","family":"Reymann","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development, Division of Infectious Diseases and Tropical Pediatrics and Division of Geographic Medicine,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Lim","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development, Division of Infectious Diseases and Tropical Pediatrics and Division of Geographic Medicine,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"179","article-title":"Distribution of mucosal IgA and IgG subclass-producing immunocytes and alterations in various disorders.","volume":"20","author":"Brandzaeg P.","year":"1986","unstructured":"Brandzaeg P. 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Egan M. L. Mestecky J. Cellular origins of human polymeric and monomeric IgA: intracellular and secreted forms of IgA. J. Immunol. 133 1984 3156 3160","journal-title":"J. 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