{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:34:42Z","timestamp":1759638882477},"reference-count":37,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study was conducted to produce a recombinant species-specific oocyst wall protein of\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            . Antigens unique to\n            <jats:italic>C. parvum<\/jats:italic>\n            were identified by gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting of oocyst proteins from several different\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species. Antiserum was then prepared against a 41-kDa antigen unique to\n            <jats:italic>C. parvum<\/jats:italic>\n            and used to identify a recombinant DNA clone, designated rCP41. Expression of CP41 mRNA in\n            <jats:italic>C. parvum<\/jats:italic>\n            oocysts was confirmed by reverse transcriptase PCR (RT-PCR). Although the CP41 sequence was shown by PCR to be present in the genome of\n            <jats:italic>C. baileyi<\/jats:italic>\n            , CP41 mRNA was not detected in this species by RT-PCR. Immunofluorescence staining with antiserum against recombinant CP41 detected native CP41 antigen on the surface of\n            <jats:italic>C. parvum<\/jats:italic>\n            oocysts but failed to detect CP41 on\n            <jats:italic>C. baileyi<\/jats:italic>\n            oocysts. Immunoelectron microscopy demonstrated that native CP41 was distributed unevenly on the\n            <jats:italic>C. parvum<\/jats:italic>\n            oocyst surface and was associated with amorphous oocyst wall material. In an enzyme-linked immunosorbent assay, purified rCP41 performed as well as native\n            <jats:italic>C. parvum<\/jats:italic>\n            oocyst protein in measuring the serological responses of young calves and adult cows to experimental and natural\n            <jats:italic>C. parvum<\/jats:italic>\n            infections. These results indicate that recombinant CP41 antigen may have potential in the immunodiagnosis of cryptosporidiosis.\n          <\/jats:p>","DOI":"10.1128\/cdli.6.6.912-920.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:24:10Z","timestamp":1577820250000},"page":"912-920","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Cloning and Expression of a DNA Sequence Encoding a 41-Kilodalton\n            <i>Cryptosporidium parvum<\/i>\n            Oocyst Wall Protein"],"prefix":"10.1128","volume":"6","author":[{"given":"Mark C.","family":"Jenkins","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Immunology and Disease Resistance Laboratory1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jim","family":"Trout","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunology and Disease Resistance Laboratory1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Murphy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Nematology Laboratory,2 Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Harp","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Metabolic Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agricultural, Ames, Iowa 500103"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jim","family":"Higgins","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunology and Disease Resistance Laboratory1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Wergin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Nematology Laboratory,2 Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Fayer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunology and Disease Resistance Laboratory1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Abrahamsen M. 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