{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T21:20:39Z","timestamp":1773868839410,"version":"3.50.1"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that \u025b and \u03b3 isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.<\/jats:p>","DOI":"10.1128\/cdli.6.6.983-985.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:24:14Z","timestamp":1577820254000},"page":"983-985","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Increased Levels of \u025b and \u03b3 Isoforms of 14-3-3 Proteins in Cerebrospinal Fluid in Patients with Creutzfeldt-Jakob Disease"],"prefix":"10.1128","volume":"6","author":[{"given":"Hidehiro","family":"Takahashi","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, National Institute of Infectious Diseases, Tokyo,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshinari","family":"Iwata","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, National Institute of Infectious Diseases, Tokyo,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, National Institute of Infectious Diseases, Tokyo,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reisuke H.","family":"Takahashi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, National Institute of Infectious Diseases, Tokyo,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Sato","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, National Institute of Infectious Diseases, Tokyo,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Enzyme Chemistry, Institute of Enzyme Research, University of Tokushima, Tokushima,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miwa","family":"Takashima","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Enzyme Chemistry, Institute of Enzyme Research, University of Tokushima, Tokushima,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kido","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Enzyme Chemistry, Institute of Enzyme Research, University of Tokushima, Tokushima,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nagashima","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, Hokkaido,3 and"},{"name":"<!--label omitted: 4-->CREST-JST, Sapporo,4 Japan, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimbra","family":"Kenney","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Clarence J.","family":"Gibbs","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kurata","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, National Institute of Infectious Diseases, Tokyo,1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/383685a0"},{"key":"e_1_3_2_3_2","unstructured":"Gajdusek\nD. C.\nInfectious amyloids: subacute spongiform encephalopathies as transmissible cerebral amyloidosis.\nFields virology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\nHowley\nP. M.\n2\n1996\nLippincott-Raven\nPhiladelphia Pa"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198607313150502"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199609263351303"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1346"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7939645"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/27938"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(97)89430-9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.121.18.419"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","unstructured":"Will R. G. J. W. Ironside M. Zeidler S. N. Cousens K. Estibeiro Alperovitch A. S. Poser M. Pocchiari A. Hofman and P. G. Smith. 1996. A new variant of Creutzfeldt-Jakob disease in the UK. Lancet 347: 921\u2013925.","DOI":"10.1016\/S0140-6736(96)91412-9"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)08077-4"},{"key":"e_1_3_2_13_2","first-page":"874","article-title":"The 14-3-3 brain protein and transmissible spongiform encephalopathy.","volume":"336","author":"Zerr I.","year":"1997","unstructured":"Zerr I. Bodemer M. Weber T. The 14-3-3 brain protein and transmissible spongiform encephalopathy. N. Engl. J. Med. 336 1997 874","journal-title":"N. Engl. J. 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