{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T12:36:16Z","timestamp":1756384576963},"reference-count":29,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two simple\n            <jats:italic>Bartonella bacilliformis<\/jats:italic>\n            immunoblot preparation methods were developed. Antigen was prepared by two different methods: sonication of whole organisms or glycine extraction. Both methods were then tested for sensitivity and specificity. Well-defined control sera were utilized in the development of these diagnostic immunoblots, and possible cross-reactions were thoroughly examined. Sera investigated for cross-reaction with these diagnostic antigens were drawn from patients with brucellosis, chlamydiosis, Q fever, and cat scratch disease, all of whom were from regions where bartonellosis is not endemic. While both immunoblots yielded reasonable sensitivity and high specificity, we recommend the use of the sonicated immunoblot, which has a higher sensitivity when used to detect acute disease and produces fewer cross-reactions. The sonicated immunoblot reported here is 94% sensitive to chronic bartonellosis and 70% sensitive to acute bartonellosis. In a healthy group, it is 100% specific. This immunoblot preparation requires a simple sonication protocol for the harvesting of\n            <jats:italic>B. bacilliformis<\/jats:italic>\n            antigens and is well suited for use in regions of endemicity.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.1.1-5.2000","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T17:52:55Z","timestamp":1240509175000},"page":"1-5","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Sonicated Diagnostic Immunoblot for Bartonellosis"],"prefix":"10.1128","volume":"7","author":[{"given":"Vania","family":"Mallqui","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Patolog\u0131\u0301a,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily C.","family":"Speelmon","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Patolog\u0131\u0301a,1"},{"name":"<!--label omitted: 2-->Asociacio\u0301n Bene\u0301fica PRISMA,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Vera\u0301stegui","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Patolog\u0131\u0301a,1"},{"name":"<!--label omitted: 3-->Departamento de Microbiolog\u0131\u0301a,3 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciro","family":"Maguin\u0303a-Vargas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Instituto de Medicina Tropical Alexander VonHumboldt,4Universidad Peruana Cayetano Heredia,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Pinell-Salles","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Asociacio\u0301n Bene\u0301fica PRISMA,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Lavarello","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Asociacio\u0301n Bene\u0301fica PRISMA,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Delgado","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Asociacio\u0301n Bene\u0301fica PRISMA,2 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Kosek","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Department of Internal Medicine, Harbor-UCLA Medical Center, Torrance, California5; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Romero","sequence":"additional","affiliation":[{"name":"<!--label omitted: 6-->United States Naval Medical Research Institute Detachment (NAMRID),6 Lima Peru;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanina","family":"Arana","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Patolog\u0131\u0301a,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Patolog\u0131\u0301a,1"},{"name":"<!--label omitted: 2-->Asociacio\u0301n Bene\u0301fica PRISMA,2 and"},{"name":"<!--label omitted: 7-->Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland7"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.354"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.57.174"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-198609000-00002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.7.1299-1302.1991"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1099\/00207713-43-4-777","article-title":"Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales.","volume":"43","author":"Brenner D. 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U.S.\n1971\nDepartment of Commerce\nSpringfield Va"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.6.766-772.1998"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.173"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.427"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.9.2270-2274.1996"},{"key":"e_1_3_2_20_2","first-page":"5","article-title":"La enfermedad de Carri\u00f3n y Leishmaniasis andina en la region de Conchucos, Distrito de Chavin, San Marcos y Huantar, Provincia de Hauri, Departamento de Ancash.","volume":"16","author":"Magui\u00f1a-Vargas C.","year":"1985","unstructured":"Magui\u00f1a-Vargas C. P\u00e9rez E. La enfermedad de Carri\u00f3n y Leishmaniasis andina en la region de Conchucos, Distrito de Chavin, San Marcos y Huantar, Provincia de Hauri, Departamento de Ancash. Diagnostico 16 1985 5 12","journal-title":"Diagnostico"},{"key":"e_1_3_2_21_2","unstructured":"Magui\u00f1a-Vargas\nC.\nLumbreras\nH.\nGotuzzo\nE.\nCrosby\nE.\nIrrivaren\nJ.\nClinical and laboratory study of patients with acute phase of Carri\u00f3n's disease in the Hospital Base Cayetano Heredia between 1969\u20131987. Publication 63.\n1988\nInternational Congress for Infectious Diseases\nRio de Janeiro Brazil"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(74)32012-5"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.10.2144-2150.1991"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.14.4545-4552.1997"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(84)90021-4"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90673-X"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(83)92032-3"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","unstructured":"Tsang\nV. C. W.\nBers\nG. E.\nHancock\nK.\nEnzyme-linked immunoelectrotransfer blot (EITB)\nEnzyme mediated immunoassay.\nNgo\nT. T.\nLenhoff\nH. 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