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BALB\/c mice inoculated with this virus produced 18-kDa protein-specific antibodies, mostly of immunoglobulin G2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein\u201318-kDa protein fusion resulted in lymphocyte proliferation and gamma interferon production. However, these mice were not protected against a challenge with the virulent strain<jats:italic>B. abortus<\/jats:italic>2308. Disruption of the 18-kDa protein's gene in vaccine strain<jats:italic>B. abortus<\/jats:italic>RB51 did not affect either the strain's protective capabilities or its in vivo attenuation characteristics. These observations suggest that the 18-kDa protein plays no role in protective immunity.<\/jats:p>","DOI":"10.1128\/cdli.7.1.114-118.2000","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T17:52:55Z","timestamp":1240509175000},"page":"114-118","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Characterization of Specific Immune Responses of Mice Inoculated with Recombinant Vaccinia Virus Expressing an 18-Kilodalton Outer Membrane Protein of<i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"7","author":[{"given":"Ramesh","family":"Vemulapalli","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio","family":"Cravero","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"},{"name":"<!--label omitted: 2-->Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, Instituo Nacional de Tecnologia Agropecuaria, Provincia Buenos Aires, Argentina2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine L.","family":"Calvert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Toth","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nammalwar","family":"Sriranganathan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Boyle","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osvaldo L.","family":"Rossetti","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, Instituo Nacional de Tecnologia Agropecuaria, Provincia Buenos Aires, Argentina2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhardt G.","family":"Schurig","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Acha P. 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