{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:33:12Z","timestamp":1791203592342,"version":"4.2.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We analyzed the serum antibody responses against two\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            fibrinogen binding proteins, the cell-bound clumping factor (Clf) and an extracellular fibrinogen binding protein (Efb). The material consisted of 105 consecutive serum samples from 41 patients suffering from\n            <jats:italic>S. aureus<\/jats:italic>\n            septicemia and 72 serum samples from healthy individuals. An enzyme-linked immunosorbent assay (ELISA) was developed. Healthy individuals showed variable levels of antibodies against the studied antigens, and cutoff levels (upper 95th percentile) against these antigens were determined. No correlation was seen between serum antibody levels against Clf and Efb. In acute-phase samples 27% of patients showed positive antibody levels against Clf and 10% showed positive levels against Efb, while in convalescent-phase samples 63% (26 of 41) showed a positive serology against Clf and 49% (20 of 41) showed a positive serology against Efb. Antibody levels against Efb were significantly lower in the acute-phase sera than in sera from healthy individuals (\n            <jats:italic>P<\/jats:italic>\n            = 0.002). An antibody response against Clf was most frequent in patients suffering from osteitis plus septic arthritis and from endocarditis (80% positive). The antibody response against Efb appeared to develop later in the course of disease. A possible biological effect of measured antibodies was demonstrated with the help of an inhibition ELISA, in which both high-titer and low-titer sera inhibited the binding of bacteria to fibrinogen. In conclusion, we have demonstrated in vivo production of\n            <jats:italic>S. aureus<\/jats:italic>\n            fibrinogen binding proteins during deep\n            <jats:italic>S. aureus<\/jats:italic>\n            infections and a possible diagnostic and prophylactic role of the corresponding serum antibodies in such infections.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.1.14-20.2000","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T17:52:55Z","timestamp":1240509175000},"page":"14-20","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Antibody Responses in Patients with Staphylococcal Septicemia against Two\n            <i>Staphylococcus aureus<\/i>\n            Fibrinogen Binding Proteins: Clumping Factor and an Extracellular Fibrinogen Binding Protein"],"prefix":"10.1128","volume":"7","author":[{"given":"Patricia","family":"Colque-Navarro","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Palma","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institute, S-141 86 Huddinge,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"So\u0308derquist","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Infectious Diseases, O\u0308rebro Medical Center Hospital, S-701 85O\u0308rebro,3 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-Ingmar","family":"Flock","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institute, S-141 86 Huddinge,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Mo\u0308llby","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Microbiology and Tumorbiology Center, Karolinska Institute, S-171 77 Stockholm,1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)92186-3"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(92)90047-R"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5433-5442.1998"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548509070419"},{"key":"e_1_3_1_6_2","first-page":"87","article-title":"Serological response to TSS toxin in Staphylococcus aureus infected patients and in healthy controls.","volume":"93","author":"Christensson B.","year":"1984","unstructured":"Christensson B. Hedstr\u00f6m S. \u00c5. Serological response to TSS toxin in Staphylococcus aureus infected patients and in healthy controls. Acta Pathol. Microbiol. Immunol. Scand. 93 1984 87 90","journal-title":"Acta Pathol. Microbiol. Immunol. Scand."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.3.530"},{"key":"e_1_3_1_8_2","first-page":"351","article-title":"Antibody response to alpha and beta hemolysin from Staphylococcus aureus in patients with staphylococcal infections and in normals.","volume":"91","author":"Christensson B.","year":"1983","unstructured":"Christensson B. Hedstr\u00f6m S. \u00c5. Kronwall G. Antibody response to alpha and beta hemolysin from Staphylococcus aureus in patients with staphylococcal infections and in normals. Acta Pathol. Microbiol. Immunol. Scand. 91 1983 351 356","journal-title":"Acta Pathol. Microbiol. Immunol. 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Dis."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1983.tb23270.x"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-44-3-171"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.4.723"},{"key":"e_1_3_1_15_2","unstructured":"Lee\nJ. C.\nPier\nB. P.\nVaccine-based strategies for prevention of Staphylococcal diseases\nThe staphylococci in human disease.\nCrossley\nK. B.\nArcher\nG. L.\n1997\n631\n654\nChurchill Livingstone\nNew York N.Y"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1994.tb00010.x"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02316.x"},{"key":"e_1_3_1_18_2","unstructured":"M\u00f6llby\nR.\nAntibody response in deep Staphylococcus aureus infections\nStaphylococci and staphylococcal infections.\nM\u00f6llby\nR.\nFlock\nJ. I.\nNord\nC. 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