{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:11:21Z","timestamp":1764688281677},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Immunoglobulin A (IgA) deficiency occurs more frequently in patients with celiac disease (CD) than in the general population and can lead to false-negative results in the best serologic test for CD, endomysial IgA (EMA). To evaluate the impact of IgA deficiency on serologic detection of CD in a reference laboratory setting, IgA levels were measured in 510 consecutive serum specimens submitted for testing for EMA; 510 consecutive serum specimens submitted for<jats:italic>Helicobacter pylori<\/jats:italic>IgG testing served as a gastrointestinal symptom control group. The frequency of IgA deficiency was significantly higher among the specimens submitted for testing for EMA (5.1%) than among the specimens from the symptom control group (1.4%). Three subsets of sera from the group of specimens submitted for testing for EMA were then tested by additional serologic assays for CD; these subsets were EMA-positive sera (<jats:italic>n<\/jats:italic>= 25), EMA-negative, IgA-deficient sera (<jats:italic>n<\/jats:italic>= 26), and control sera (from EMA-negative, IgA-nondeficient patients age matched to IgA-deficient patients;<jats:italic>n<\/jats:italic>= 26). The proportions of EMA-positive sera positive by other assays for CD were 92% for transglutaminase IgA (TG-IgA), 80% for gliadin IgA, 84% for gliadin IgG, 60% for endomysial IgG (EMG), and 32% for transglutaminase IgG (TG-IgG). Very low proportions (0 to 8%) of IgA-deficient sera and control sera were positive for TG-IgA, gliadin IgA, EMG, and TG-IgG. Eight of 26 (31%) IgA-deficient serum samples were positive for gliadin IgG, whereas 3 of 26 (12%) control serum samples were positive for gliadin IgG, but this difference was not statistically significant. Physicians supplied clinical data for 18 of 26 patients with IgA deficiency; only 4 patients had undergone small-bowel biopsy, and 0 of 4 patients showed villous atrophy. These findings show that IgA deficiency is found more frequently among sera submitted for testing for EMA in a reference laboratory setting, but there was no clear-cut serologic or clinical evidence of CD in EMA-negative, IgA-deficient patients.<\/jats:p>","DOI":"10.1128\/cdli.7.2.192-196.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"192-196","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Immunoglobulin A (IgA) Deficiency and Alternative Celiac Disease-Associated Antibodies in Sera Submitted to a Reference Laboratory for Endomysial IgA Testing"],"prefix":"10.1128","volume":"7","author":[{"given":"Harry E.","family":"Prince","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->MRL Reference Laboratory, Cypress, California,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary L.","family":"Norman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->INOVA Diagnostics, San Diego, California2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter L.","family":"Binder","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->INOVA Diagnostics, San Diego, California2"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1998.0253"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)92352-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)61732-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00441577"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.3.362"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.73.1.3"},{"key":"e_1_3_2_8_2","first-page":"527","article-title":"Effectiveness of antigliadin antibodies as a screening test for celiac disease in children.","volume":"157","author":"Chartrand L. 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