{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:34:01Z","timestamp":1790854441684,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The 17-kDa antigen of\n            <jats:italic>Bartonella henselae<\/jats:italic>\n            has previously been shown to elicit a strong humoral immune response in patients with cat scratch disease (CSD) and to be useful in screening human serum samples for CSD. In this study, PCR amplification of genes homologous to the 17-kDa antigen gene of\n            <jats:italic>B. henselae<\/jats:italic>\n            was performed using genomic DNAs from several species of\n            <jats:italic>Bartonella<\/jats:italic>\n            , including the currently recognized human pathogens. Amplicons of similar size were demonstrated using the following chromosomal DNA templates:\n            <jats:italic>B. henselae<\/jats:italic>\n            (two strains),\n            <jats:italic>B. quintana<\/jats:italic>\n            (two strains),\n            <jats:italic>B. elizabethae<\/jats:italic>\n            ,\n            <jats:italic>B. clarridgeiae<\/jats:italic>\n            ,\n            <jats:italic>B. vinsonii<\/jats:italic>\n            subsp.\n            <jats:italic>vinsonii<\/jats:italic>\n            , and\n            <jats:italic>B. vinsonii<\/jats:italic>\n            subsp.\n            <jats:italic>berkhoffii<\/jats:italic>\n            . No evidence of a\n            <jats:italic>B. bacilliformis<\/jats:italic>\n            homolog of the 17-kDa antigen gene was obtained using multiple primer pairs. DNA sequencing revealed open reading frames capable of coding for proteins with sizes similar to that of the 17-kDa antigen of\n            <jats:italic>B. henselae<\/jats:italic>\n            in all of the amplicons; however, extensive sequence divergence across the genus was noted. Cloning of the amplified products into pUC19 resulted in recombinants that directed synthesis of homologs of the 17-kDa protein. Immunoblot analysis using human sera from CSD cases demonstrated very little cross-reactivity among different species for this protein. In contrast, immunoblots using rabbit serum raised to the recombinant\n            <jats:italic>B. henselae<\/jats:italic>\n            antigen showed extensive cross-reactivity with the proteins of other\n            <jats:italic>Bartonella<\/jats:italic>\n            species. The data suggest that the use of the 17-kDa antigen as a serologic reagent may allow the development of more specific diagnostic assays. Furthermore, the nucleotide sequences from the various versions of the 17-kDa antigen gene should be useful for rapid identification of\n            <jats:italic>Bartonella<\/jats:italic>\n            at the species level.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.2.251-257.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"251-257","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Conservation of the 17-Kilodalton Antigen Gene within the Genus\n            <i>Bartonella<\/i>"],"prefix":"10.1128","volume":"7","author":[{"given":"Debra","family":"Sweger","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 33612,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Resto-Ruiz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 33612,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P.","family":"Johnson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Infectious Diseases, Bay Pines Veterans Affairs Medical Center, Bay Pines, Florida 335042"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Schmiederer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 33612,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noel","family":"Hawke","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 33612,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burt","family":"Anderson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 33612,1 and"},{"name":"<!--label omitted: 2-->Division of Infectious Diseases, Bay Pines Veterans Affairs Medical Center, Bay Pines, Florida 335042"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.354"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.3.2.200-204.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00402.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00330-7"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.9.2358-2365.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.2.203"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.4.942-948.1994"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.6.1503"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1931-1937.1997"},{"key":"e_1_3_2_11_2","first-page":"261","article-title":"Differentiation of Bartonella species using restriction endonuclease analysis of PCR-amplified 16S rRNA genes.","volume":"129","author":"Birtles R. J.","year":"1995","unstructured":"Birtles R. J. Differentiation of Bartonella species using restriction endonuclease analysis of PCR-amplified 16S rRNA genes. FEMS Microbiol. Lett. 129 1995 261 265","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-1-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00580-5"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.154-160.1995"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1099\/00207713-43-4-777","article-title":"Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov. Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales.","volume":"43","author":"Brenner D. J.","year":"1993","unstructured":"Brenner D. J. O'Connor S. P. Winkler H. H. Steigerwalt A. G. Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov. Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int. J. Syst. Bacteriol. 43 1993 777 786","journal-title":"Int. J. Syst. Bacteriol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.8.2107-2113.1995"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1995.00430150164017"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.4.872-881.1993"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3109\/10520296409061219"},{"key":"e_1_3_2_20_2","first-page":"109","article-title":"Techniques for transformation of E. coli","volume":"1","author":"Hanahan D.","year":"1985","unstructured":"Hanahan D. Techniques for transformation of E. coli DNA cloning: a practical approach Glover D. M. 1 1985 109 136 IRL Press Oxford, United Kingdom","journal-title":"DNA cloning: a practical approach"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-4-1333"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-1-283"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90330-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/137.5.578"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3824"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00469.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.83.12.1707"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.427"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1813-1818.1997"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-3-704"},{"key":"e_1_3_2_31_2","first-page":"64","article-title":"Description of Bartonella clarridgeiae sp. nov. isolated from the cat of a patient with Bartonella henselae septicemia.","volume":"5","author":"Lawson P. A.","year":"1996","unstructured":"Lawson P. A. Collins M. D. Description of Bartonella clarridgeiae sp. nov. isolated from the cat of a patient with Bartonella henselae septicemia. Med. Microbiol. Lett. 5 1996 64 73","journal-title":"Med. Microbiol. Lett."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.3.683"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1797-1803.1995"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/19.2.355"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.2.265-274.1992"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92032-B"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1992.00400150114021"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.96.6.1137"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-32-3-305"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2598-2601.1999"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199307013290102"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1220"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.2.251-257.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.2.251-257.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:28:05Z","timestamp":1645590485000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.7.2.251-257.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1128\/CDLI.7.2.251-257.2000"],"URL":"https:\/\/doi.org\/10.1128\/cdli.7.2.251-257.2000","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]},"assertion":[{"value":"1999-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-12-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}