{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:32:39Z","timestamp":1761154359664},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Strain variations of\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whether\n            <jats:italic>H. pylori<\/jats:italic>\n            expresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE).\n            <jats:italic>H. pylori<\/jats:italic>\n            strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in the\n            <jats:italic>H. pylori<\/jats:italic>\n            genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation that\n            <jats:italic>H. pylori<\/jats:italic>\n            exhibits.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.2.301-306.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:53Z","timestamp":1027764233000},"page":"301-306","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Clustering of Clinical Strains of\n            <i>Helicobacter pylori<\/i>\n            Analyzed by Two-Dimensional Gel Electrophoresis"],"prefix":"10.1128","volume":"7","author":[{"given":"Helena","family":"Enroth","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Epidemiology, Karolinska Institute,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"\u212bkerlund","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Bacteriology, Swedish Institute for Infectious Disease Control,2 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Sille\u0301n","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Bacteriology, Swedish Institute for Infectious Disease Control,2 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Engstrand","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Epidemiology, Karolinska Institute,1 and"},{"name":"<!--label omitted: 2-->Department of Bacteriology, Swedish Institute for Infectious Disease Control,2 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"181","article-title":"Electrophoretic analysis of proteins from Mycoplasma hominis strains detected by SDS-PAGE, two-dimensional gel electrophoresis and immunoblotting.","volume":"133","author":"Andersen H.","year":"1987","unstructured":"Andersen H. 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