{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T04:27:18Z","timestamp":1783744038895,"version":"3.55.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A new meningococcal group C-CRM<jats:sub>197<\/jats:sub>conjugate vaccine (MnCC; Meningitec) has been evaluated in multiple clinical trials in the United States and most recently has been approved for routine administration in the United Kingdom. Meningococcal serogroup C (MnC)-specific immunoglobulin G (IgG) antibodies in pre- and postimmunization sera obtained from healthy U.S. adults, toddlers, and infants were quantitated by enzyme-linked immunosorbent assay (ELISA) and by an antibody-dependent, complement-mediated serum bactericidal assay (SBA). Serogroup-specific IgG antibody (micrograms per milliliter) in adults immunized either with the quadrivalent polysaccharide (A, C, Y, and W-135) vaccine or with MnCC showed a strong correlation (<jats:italic>r<\/jats:italic>= 0.848 and 0.934, respectively) by linear regression analysis with SBA. Sera from infants immunized with the MnCC (<jats:italic>n<\/jats:italic>= 30) and an age-matched unimmunized control group (<jats:italic>n<\/jats:italic>= 15) were also analyzed. Linear regression analysis of serum bactericidal and IgG ELISA data from sera obtained at 2 months of age (preimmunization) showed no correlation; however, a high degree of correlation was observed at time points after two (<jats:italic>r<\/jats:italic>= 0.877) and three (<jats:italic>r<\/jats:italic>= 0.951) immunizations, where significant rises in anti-MnC polysaccharide antibodies occurred relative to the age-matched control group. Infants previously primed with 3 doses of MnCC were given a booster dose of conjugate vaccine at 12 to 15 months of age. The correlation coefficient of ELISA to SBA for combined pre- and postbooster data was<jats:italic>r<\/jats:italic>= 0.836 (<jats:italic>n<\/jats:italic>= 48 pairs). In conclusion, increases in serum bactericidal activity in immunized adult, toddler, and infant populations were found to correlate very well with increases in serogroup-specific IgG concentrations, whereas the correlation between these two assays in nonimmunized 2-month-old infants was poor. Characterizing the relationship between these methods is important for understanding the significance of antigen-specific antibody concentrations relative to vaccine performance and protection from disease.<\/jats:p>","DOI":"10.1128\/cdli.7.5.764-768.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"764-768","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Relationship between Serum Bactericidal Activity and Serogroup-Specific Immunoglobulin G Concentration for Adults, Toddlers, and Infants Immunized with<i>Neisseria meningitidis<\/i>Serogroup C Vaccines"],"prefix":"10.1128","volume":"7","author":[{"given":"Daniel J.","family":"Sikkema","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith E.","family":"Friedman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartholomew","family":"Corsaro","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Kimura","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen W.","family":"Hildreth","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dace V.","family":"Madore","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally A.","family":"Quataert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1996.tb14182.x"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197002122820701"},{"key":"e_1_3_1_4_2","first-page":"8","article-title":"Recommended childhood immunization schedule\u2014United States, 1998.","volume":"47","author":"Centers for Disease Control and Prevention","year":"1998","unstructured":"Centers for Disease Control and Prevention Recommended childhood immunization schedule\u2014United States, 1998. 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