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To determine whether local humoral and cytokine responses are related to each other and to histologic responses, we studied 66 Japanese patients who underwent gastroscopy. Using specific enzyme-linked immunosorbent assays, we examined gastric antral mucosal-organ biopsy culture supernatants to assess interleukin-6 (IL-6) and interleukin-8 (IL-8) levels and antibody responses to<jats:italic>H. pylori<\/jats:italic>whole-cell antigens CagA, HspA, and HspB. Of the patients studied, 11 were<jats:italic>H. pylori<\/jats:italic>negative and 55 were<jats:italic>H. pylori<\/jats:italic>positive; by PCR, all strains were<jats:italic>cagA<\/jats:italic><jats:sup>+<\/jats:sup>. As expected, compared to<jats:italic>H. pylori<\/jats:italic>-negative patients,<jats:italic>H. pylori<\/jats:italic>-positive patients had significantly higher humoral responses to all<jats:italic>H. pylori<\/jats:italic>antigens and had higher IL-8 (47.8 \u00b1 3.5 versus 10.1 \u00b1 4.3 ng\/mg of biopsy protein;<jats:italic>P<\/jats:italic>&lt; 0.001) and IL-6 levels (2.8 \u00b1 0.3 versus 0.26 \u00b1 0.2 ng\/mg of protein;<jats:italic>P<\/jats:italic>&lt; 0.001). Among the<jats:italic>H. pylori<\/jats:italic>-positive patients, supernatant anti-CagA immunoglobulin G (IgG) levels were significantly associated with<jats:italic>H. pylori<\/jats:italic>density (<jats:italic>P<\/jats:italic>&lt; 0.005) and neutrophil infiltration (<jats:italic>P<\/jats:italic>&lt; 0.005) scores. Anti-CagA immunoglobulin A levels were correlated with intestinal metaplasia (<jats:italic>P<\/jats:italic>&lt; 0.05). Mononuclear cell infiltration scores were significantly associated with supernatant IL-6 levels (<jats:italic>P<\/jats:italic>&lt; 0.005) and with IgG responses to whole-cell antigens (<jats:italic>P<\/jats:italic>&lt; 0.05). Supernatant IL-8 levels were significantly associated with anti-CagA IgG (<jats:italic>r<\/jats:italic>= 0.75,<jats:italic>P<\/jats:italic>&lt; 0.001). Anti-CagA responses correlated with neutrophil infiltration, intestinal metaplasia,<jats:italic>H. pylori<\/jats:italic>density, and IL-8 levels, suggesting that the absolute levels of these antibodies may be markers for gastric inflammation and premalignant changes in individual hosts.<\/jats:p>","DOI":"10.1128\/cdli.7.5.803-809.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"803-809","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Anti-CagA Immunoglobulin G Responses Correlate with Interleukin-8 Induction in Human Gastric Mucosal Biopsy Culture"],"prefix":"10.1128","volume":"7","author":[{"given":"Takafumi","family":"Ando","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Disease, Department of Medicine, Vanderbilt University School of Medicine,1 and"},{"name":"<!--label omitted: 2-->First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo I.","family":"Perez-Perez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Disease, Department of Medicine, Vanderbilt University School of Medicine,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Kusugami","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Ohsuga","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen C.","family":"Bloch","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Disease, Department of Medicine, Vanderbilt University School of Medicine,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin J.","family":"Blaser","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Disease, Department of Medicine, Vanderbilt University School of Medicine,1 and"},{"name":"<!--label omitted: 3-->VA Medical Center,3 Nashville, Tennessee, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.10.4742-4747.1998"},{"key":"e_1_3_2_3_2","first-page":"1150","article-title":"Interleukin-8 activity correlates with histological severity in Helicobacter pylori-associated antral gastritis.","volume":"91","author":"Ando T.","year":"1996","unstructured":"Ando T. 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