{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:06:36Z","timestamp":1762520796992,"version":"build-2065373602"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A new immunoenzymatic test, named the thin-layer immunoassay\u2013enzyme-linked immunosorbent assay (TIA-ELISA), was evaluated for antibody detection in human trichinellosis using excretion and secretion products prepared from\n            <jats:italic>Trichinella spiralis<\/jats:italic>\n            muscle larvae. Serum samples from people with positive muscle biopsies or symptoms compatible with the disease (\n            <jats:italic>n<\/jats:italic>\n            = 8 or 26, respectively), all reactive in enzyme-linked immunoelectrotransfer blot assay (EITB), as well as 67 serum samples from healthy, EITB-negative people, were tested in an ELISA and TIA-ELISA. TIA-ELISA was performed in polystyrene plastic petri dishes by adding dots of 10 \u03bcl each of antigen (7 \u03bcg\/ml) followed by adding diluted serum and the conjugate. Finally, the substrate mixed with agar was added to develop the reaction. Enzymatic by-products were easily detected by the naked eye as defined dots. Sensitivity and specificity were 76 and 94% for ELISA, and both parameters were 91% for TIA-ELISA. The kappa correlation indices for both tests in relation to EITB were 0.73 and 0.80, respectively. The TIA-ELISA can be carried out with common laboratory equipment in 3 h and uses lower quantities of antigen than EITB and ELISA. Since TIA-ELISA is easy to perform, cheap, sensitive, and specific, the test could be an acceptable alternative to use in clinical laboratories lacking specialized equipment needed for ELISA and EITB and in field studies for antibody detection in human trichinellosis.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.5.810-812.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"810-812","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Serological Evaluation of Thin-Layer Immunoassay\u2013Enzyme-Linked Immunosorbent Assay for Antibody Detection in Human Trichinellosis"],"prefix":"10.1128","volume":"7","author":[{"given":"Alberto","family":"Go\u0301mez-Priego","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Zoonosis, Instituto Nacional de Diagno\u0301stico y Referencia Epidemiolo\u0301gicos, Secretar\u0131\u0301a de Salud,1 and"},{"name":"<!--label omitted: 2-->Departamento de Microbiolog\u0131\u0301a y Parasitolog\u0131\u0301a, Facultad de Medicina, Universidad Nacional Auto\u0301noma de Me\u0301xico,2 Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidia","family":"Crecencio-Rosales","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Zoonosis, Instituto Nacional de Diagno\u0301stico y Referencia Epidemiolo\u0301gicos, Secretar\u0131\u0301a de Salud,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge-Luis","family":"de-la-Rosa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Zoonosis, Instituto Nacional de Diagno\u0301stico y Referencia Epidemiolo\u0301gicos, Secretar\u0131\u0301a de Salud,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90173-V"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","unstructured":"Altman\nD. G.\nPractical statistics for medical research.\n1991\nChapman and Hall\nLondon England","DOI":"10.1201\/9780429258589"},{"key":"e_1_3_2_4_2","first-page":"103","article-title":"Use of purified surface\/stichosomal antigens of Trichinella spiralis muscle larvae in the detection of naturally infected swine.","volume":"51","author":"Arriaga C.","year":"1991","unstructured":"Arriaga C. Salinas-Tob\u00f3n R. Morilla A. Ortega-Pierres G. Use of purified surface\/stichosomal antigens of Trichinella spiralis muscle larvae in the detection of naturally infected swine. Res. Rev. Parasitol. 51 1991 103 105","journal-title":"Res. Rev. Parasitol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282258"},{"key":"e_1_3_2_6_2","first-page":"205","article-title":"Outbreak of trichinellosis associated with eating cougar jerky, Idaho, 1995.","volume":"45","author":"Centers for Disease Control and Prevention","year":"1996","unstructured":"Centers for Disease Control and Prevention Outbreak of trichinellosis associated with eating cougar jerky, Idaho, 1995. Morbid Mortal. Wkly. Rep. 45 1996 205 206","journal-title":"Morbid Mortal. Wkly. Rep."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/16.4.500"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.2.1.122-124.1995"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00163-X"},{"key":"e_1_3_2_10_2","unstructured":"de-la-Rosa\nJ. L.\nCorrea\nD.\nLongitudinal analysis of the humoral immune response of rats experimentally infected with Trichinella spiralis by western blot\nProceedings of the Ninth International Conference on Trichinellosis.\nOrtega-Pierres\nG.\nGamble\nH. R.\nvan-Knapen\nF.\nWakelin\nD.\n1996\n333\n337\nCentro de Investigaci\u00f3n y Estudios Avanzados del Instituto Polit\u00e9cnico Nacional\nMexico City Mexico"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","unstructured":"Despommier\nD. D.\nBiology\nTrichinella and trichinosis.\nCampbell\nW. C.\n1983\n75\n142\nPlenum Press\nNew York N.Y","DOI":"10.1007\/978-1-4613-3578-8_3"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00151717"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(77)90084-9"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(79)90290-4"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(83)90051-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90321-9"},{"key":"e_1_3_2_17_2","first-page":"353","article-title":"DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection.","volume":"24","author":"G\u00f3mez-Priego A.","year":"1993","unstructured":"G\u00f3mez-Priego A. Cruz-Guti\u00e9rrez L. E. Paniagua-Sol\u0131\u0301s J. F. Ruenes-Meza M. T. DIG-ELISA test in onchocerciasis. Serum antibodies and probability of infection. Arch. Med. Res. 24 1993 353 359","journal-title":"Arch. Med. Res."},{"key":"e_1_3_2_18_2","first-page":"45","article-title":"Triquinosis aguda 1992: epidemia de 166 casos en Ciudad Delicias, Chih. Diagn\u00f3stico por compresi\u00f3n tisular y tinci\u00f3n.","volume":"128","author":"Hern\u00e1ndez M.","year":"1992","unstructured":"Hern\u00e1ndez M. Ramos M. E. Casco S. E. J. Morales G. J. M. P\u00e9rez M. J. M. Triquinosis aguda 1992: epidemia de 166 casos en Ciudad Delicias, Chih. Diagn\u00f3stico por compresi\u00f3n tisular y tinci\u00f3n. Gac. Med. Mex. 128 1992 45 50","journal-title":"Gac. Med. Mex."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931651"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282932"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/107.1.65"},{"key":"e_1_3_2_22_2","unstructured":"Lambillotte\nD.\nGamble\nH. R.\nEvaluation of rapid ELISA for the detection of serum antibodies in swine to Trichinella spiralis Proceedings of the Ninth International Conference on Trichinellosis.\nOrtega-Pierres\nG.\nGamble\nH. R.\nvan-Knapen\nF.\nWakelin\nD.\n1996\n425\n428\nCentro de Investigaci\u00f3n y Estudios Avanzados del Instituto Polit\u00e9cnico Nacional\nMexico City Mexico"},{"key":"e_1_3_2_23_2","unstructured":"Murrell\nK. D.\nBruschi\nF.\nClinical trichinellosis\nProgress in clinical parasitology.\nSun\nT.\n1994\n117\n150\nCRC Press Inc.\nBoca Raton Fla"},{"key":"e_1_3_2_24_2","first-page":"23","article-title":"Detecci\u00f3n de anticuerpos en la oncocercosis humana con DIG-ELISA usando un extracto crudo de Onchocerca gutturosa.","volume":"2","author":"Semba R. D.","year":"1989","unstructured":"Semba R. D. Rivas-Alcal\u00e1 A. R. G\u00f3mez-Priego A. Detecci\u00f3n de anticuerpos en la oncocercosis humana con DIG-ELISA usando un extracto crudo de Onchocerca gutturosa. Rev. Mex. 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Parazytol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282561"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.53.241"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(81)90328-8"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.5.810-812.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.5.810-812.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:59:03Z","timestamp":1645592343000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.7.5.810-812.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1128\/CDLI.7.5.810-812.2000"],"URL":"https:\/\/doi.org\/10.1128\/cdli.7.5.810-812.2000","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"type":"print","value":"1071-412X"},{"type":"electronic","value":"1098-6588"}],"subject":[],"published":{"date-parts":[[2000,9]]},"assertion":[{"value":"2000-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-05-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}