{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T05:24:47Z","timestamp":1770528287229,"version":"3.49.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which\n            <jats:italic>Brucella suis<\/jats:italic>\n            was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98.3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which\n            <jats:italic>B. suis<\/jats:italic>\n            was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.5.828-831.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"828-831","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina"],"prefix":"10.1128","volume":"7","author":[{"given":"P. Silva","family":"Paulo","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Vigliocco","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. F.","family":"Ramondino","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Marticorena","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bissi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Briones","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gorchs","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Gall","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P92"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P92"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Alton\nG. G.\nJones\nL. M.\nAngus\nR. D.\nVerger\nJ. M.\nTechniques for the brucellosis laboratory\n1988\n13\n61\nInstitut National de la Recherche Agronomique\nParis France"},{"key":"e_1_3_2_3_2","first-page":"349","article-title":"The production and evaluation of a buffered plate antigen for use in a presumptive test for brucellosis.","volume":"56","author":"Angus R. D.","year":"1984","unstructured":"Angus R. D. Barton C. E. The production and evaluation of a buffered plate antigen for use in a presumptive test for brucellosis. Dev. Biol. Stand. 56 1984 349 356","journal-title":"Dev. Biol. Stand."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/sim.4780060402"},{"key":"e_1_3_2_5_2","first-page":"927","article-title":"Recent advances in the study of Brucella antigens and their serological cross reactions.","volume":"55","author":"Corbel M. J.","year":"1985","unstructured":"Corbel M. J. Recent advances in the study of Brucella antigens and their serological cross reactions. Vet. Bull. 55 1985 927 941","journal-title":"Vet. Bull."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1935(84)90055-1"},{"key":"e_1_3_2_7_2","unstructured":"Deyoe\nB. L.\nDiseases of swine\n6th ed.\nLeman\nA. D.\nStraw\nB.\nGlock\nR. D.\nMengelin\nW. L.\nPenny\nR. H. C.\nScholl\nE.\n1987\n Iowa State University Press\nAmes"},{"key":"e_1_3_2_8_2","first-page":"99","volume":"4","author":"Garcia Carrillo C.","year":"1971","unstructured":"Garcia Carrillo C. Cedro V. De Benedetti L. Evaluaci\u00f3n de t\u00e9cnicas serol\u00f3gicas en cerdos con infecci\u00f3n reciente de Brucella suis. serie 4, vol. VIII, no. 4 1971 99 107 Revista de Investigaciones Agropecuarias, INTA Republica Argentina","journal-title":"Evaluaci\u00f3n de t\u00e9cnicas serol\u00f3gicas en cerdos con infecci\u00f3n reciente de Brucella suis."},{"key":"e_1_3_2_9_2","first-page":"5","article-title":"Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19.","volume":"50","author":"Nielsen K.","year":"1989","unstructured":"Nielsen K. Cherwonogrodzky J. W. Duncan R. J. Bundle D. R. Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19. Am. J. Vet. Res. 50 1989 5 9","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(90)90007-F"},{"key":"e_1_3_2_11_2","unstructured":"Nielsen\nK.\nGall\nD.\nKelly\nW. A.\nHenning\nD.\nGarcia\nM.\nEnzyme immunoassay: application to diagnosis of bovine brucellosis.\n1992\nAgriculture Canada\nNepean Canada"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)05361-U"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","unstructured":"Nielsen\nK.\nGall\nD.\nKelly\nA.\nVigliocco\nA.\nHenning\nD.\nGarcia\nM.\nImmunoassay development\u2014application to enzyme immunoassays for the diagnosis of brucellosis.\n1996\nAgriculture and Agri-Food Canada\nNepean Canada","DOI":"10.1016\/0167-5877(95)00513-7"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(96)00116-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00195-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00077-2"},{"key":"e_1_3_2_17_2","unstructured":"Office International des Epizooties\nManual of standards for diagnostic tests and vaccines\n1996\n471\nOffice International des Epizooties\nParis France"},{"key":"e_1_3_2_18_2","first-page":"967","article-title":"Development of an enzyme-linked immunosorbent assay (ELISA) for detecting antibody to Brucella suis in porcine sera, abstr. 7528.","volume":"56","author":"Priadi A.","year":"1986","unstructured":"Priadi A. Casanah V. Hirst R. G. Emmins J. J. van der Giessen J. Soeroso M. Development of an enzyme-linked immunosorbent assay (ELISA) for detecting antibody to Brucella suis in porcine sera, abstr. 7528. Vet. Bull. 56 1986 967 (Abstract.)","journal-title":"Vet. Bull."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-2607(95)01703-8"},{"key":"e_1_3_2_20_2","unstructured":"Servicio Nacional de Sanidad Animal\nProcedure manual: serological diagnosis of bovine brucellosis.\n1993\nServicio Nacional de Sanidad Animal.\nBuenos Aires Argentina"},{"key":"e_1_3_2_21_2","first-page":"294","article-title":"Development of an enzyme-linked immunosorbent assay for detecting antibodies in sera of Brucella suis infected swine.","volume":"44","author":"Thoen C. O.","year":"1980","unstructured":"Thoen C. O. Hopkins M. P. Armbrust A. L. Angus R. D. Pietz D. E. Development of an enzyme-linked immunosorbent assay for detecting antibodies in sera of Brucella suis infected swine. Can. J. Comp. Med. 44 1980 294 298","journal-title":"Can. J. Comp. Med."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(84)90052-X"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.132.18.449"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.5.828-831.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.5.828-831.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:57:00Z","timestamp":1645592220000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.7.5.828-831.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1128\/CDLI.7.5.828-831.2000"],"URL":"https:\/\/doi.org\/10.1128\/cdli.7.5.828-831.2000","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]},"assertion":[{"value":"1999-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-05-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}