{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:31Z","timestamp":1790873131689,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            DNA polymorphism of the\n            <jats:italic>bp26<\/jats:italic>\n            gene, coding for a diagnostic protein antigen for brucellosis, was assessed by PCR and restriction fragment length polymorphism analysis using primers to amplify the\n            <jats:italic>bp26<\/jats:italic>\n            gene with its flanking regions. Surprisingly, whereas PCR performed on DNA of the reference strains of the six recognized\n            <jats:italic>Brucella<\/jats:italic>\n            species produced a product of the expected size (1,029 bp), PCR performed on DNA of three representative strains from marine mammals (from a seal, a dolphin, and a porpoise) produced a larger product, of about 1,900 bp. Nucleotide sequencing of the 1,900-bp PCR products revealed the presence of an insertion sequence, IS\n            <jats:italic>711<\/jats:italic>\n            , downstream of the\n            <jats:italic>bp26<\/jats:italic>\n            gene and adjacent to a Bru-RS1 element previously described as being a hot spot for IS\n            <jats:italic>711<\/jats:italic>\n            insertion. PCR performed on a large number of field strains from different geographic origins and from marine mammal isolates indicated that the occurrence of an IS\n            <jats:italic>711<\/jats:italic>\n            element downstream of the\n            <jats:italic>bp26<\/jats:italic>\n            gene was a feature specific to the marine mammal\n            <jats:italic>Brucella<\/jats:italic>\n            strains. Thus, this PCR assay is able to differentiate\n            <jats:italic>Brucella<\/jats:italic>\n            terrestrial isolates from marine mammal isolates and could be applied for diagnostic purposes.\n          <\/jats:p>","DOI":"10.1128\/cdli.7.5.835-839.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"835-839","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["An IS\n            <i>711<\/i>\n            Element Downstream of the\n            <i>bp26<\/i>\n            Gene Is a Specific Marker of\n            <i>Brucella<\/i>\n            spp. Isolated from Marine Mammals"],"prefix":"10.1128","volume":"7","author":[{"given":"Axel","family":"Cloeckaert","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maggy","family":"Grayon","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Grepinet","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.10.4603-4607.1988"},{"key":"e_1_3_2_3_2","unstructured":"Alton\nG. G.\nJones\nL. M.\nAngus\nR. D.\nVerger\nJ. M.\nTechniques for the brucellosis laboratory.\n1988\nInstitut National de la Recherche Agronomique\nParis France"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1258-1262.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-12-3267"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08327.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-9-2111"},{"key":"e_1_3_2_9_2","first-page":"377","article-title":"Genus Brucella Meyer and Shaw 1920, 173AL","volume":"1","author":"Corbel M. J.","year":"1984","unstructured":"Corbel M. J. Brinley-Morgan W. J. Genus Brucella Meyer and Shaw 1920, 173AL Bergey's manual of systematic bacteriology Krieg N. R. Holt J. 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CRC Press Boca Raton Fla."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879400600408"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.23.7778-7783.1992"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00688.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.138.24.583"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(92)90034-L"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01306.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90236-V"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.12.6637-6640.1990"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00118-1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2490-2499.1996"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.5.1299-1302.1996"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-12-3265"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.138.26.647"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.165-169.1996"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.5.760-764.1999"},{"key":"e_1_3_2_26_2","unstructured":"Verger J. M. M. Grayon A. Cloeckaert M. Lef\u00e8vre E. Ageron and F. Grimont. Classification of Brucella strains isolated from marine mammals using DNA-DNA hybridization and ribotyping. Res. Microbiol. in press."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-35-3-292"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-9-2913"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.5.835-839.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.7.5.835-839.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:59:20Z","timestamp":1645592360000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.7.5.835-839.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1128\/CDLI.7.5.835-839.2000"],"URL":"https:\/\/doi.org\/10.1128\/cdli.7.5.835-839.2000","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]},"assertion":[{"value":"2000-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-06-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}