{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T06:19:32Z","timestamp":1794032372179,"version":"4.8.0"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Antibodies against dengue virus type 2 and 4 proteins in acute-phase sera of 10 primary and 10 secondary dengue fever and dengue hemorrhagic fever patients were studied by Western blotting. In the first group the immune response was barely detectable, while in the second group more proteins were detected, with a very strong reaction. Anti-NS1 and -NS3 antibodies were detected mainly in secondary cases. Anti-E, -NS3, and -NS5 antibodies were detected in a high number of cases. The possibility of implementing early diagnostic assays for antigen detection is suggested.<\/jats:p>","DOI":"10.1128\/cdli.7.5.856-857.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"856-857","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":85,"title":["Human Dengue Antibodies against Structural and Nonstructural Proteins"],"prefix":"10.1128","volume":"7","author":[{"given":"Katia","family":"Valde\u0301s","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Virology, PAHO\/WHO Collaborating Center for Viral Diseases, \u201cPedro Kouri\u201d Tropical Medicine Institute, Autopista Novia del Mediod\u0131\u0301a, Marianao 13, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayling","family":"Alvarez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Virology, PAHO\/WHO Collaborating Center for Viral Diseases, \u201cPedro Kouri\u201d Tropical Medicine Institute, Autopista Novia del Mediod\u0131\u0301a, Marianao 13, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maritza","family":"Pupo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Virology, PAHO\/WHO Collaborating Center for Viral Diseases, \u201cPedro Kouri\u201d Tropical Medicine Institute, Autopista Novia del Mediod\u0131\u0301a, Marianao 13, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Va\u0301zquez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Virology, PAHO\/WHO Collaborating Center for Viral Diseases, \u201cPedro Kouri\u201d Tropical Medicine Institute, Autopista Novia del Mediod\u0131\u0301a, Marianao 13, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayner","family":"Rodri\u0301guez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Virology, PAHO\/WHO Collaborating Center for Viral Diseases, \u201cPedro Kouri\u201d Tropical Medicine Institute, Autopista Novia del Mediod\u0131\u0301a, Marianao 13, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a G.","family":"Guzma\u0301n","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Virology, PAHO\/WHO Collaborating Center for Viral Diseases, \u201cPedro Kouri\u201d Tropical Medicine Institute, Autopista Novia del Mediod\u0131\u0301a, Marianao 13, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.44.481"},{"key":"e_1_3_2_3_2","unstructured":"Falconar\nA. K. J.\nPh.D. thesis.\n1992\nLondon School of Hygiene and Tropical Medicine\nLondon United Kingdom"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.239.4839.476"},{"key":"e_1_3_2_5_2","unstructured":"Innis\nB. L.\nAntibody responses to dengue virus infection\nDengue and dengue hemorrhagic fever\u20141997.\nGubler\nD.\nKuno\nG.\n1987\n221\n243\nCAB International\nNew York N.Y"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890320207"},{"key":"e_1_3_2_7_2","unstructured":"Kurane\nI.\nEnnis\nF. A.\nImmunopathogenesis of dengue virus infection\nDengue and dengue hemorrhagic fever\u20141997.\nGubler\nD.\nKuno\nG.\n1997\n273\n290\nCAB International\nNew York N.Y"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_9_2","unstructured":"Pan American Health Organization\nDengue and dengue hemorrhagic fever in the Americas: guidelines for prevention and control. Scientific publication 548.\n1994\nPan American Health Organization\nWashington D.C."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199903)57:3<322::AID-JMV17>3.0.CO;2-5"},{"key":"e_1_3_2_11_2","first-page":"108","article-title":"ELISA de inhibici\u00f3n. Su utilidad para clasificar un caso de dengue.","volume":"49","author":"V\u00e1zquez S.","year":"1997","unstructured":"V\u00e1zquez S. Bravo J. R. P\u00e9rez A. B. Guzm\u00e1n M. G. ELISA de inhibici\u00f3n. Su utilidad para clasificar un caso de dengue. Rev. Cub. Med. Trop. 49 1997 108 112","journal-title":"Rev. Cub. Med. 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