{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:30:25Z","timestamp":1759048225868},"reference-count":9,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The efficacy of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            RB51 and hot saline extract (HSE) from\n            <jats:italic>Brucella ovis<\/jats:italic>\n            as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with\n            <jats:italic>B. abortus<\/jats:italic>\n            strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 \u00d7 10\n            <jats:sup>9<\/jats:sup>\n            CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detect\n            <jats:italic>B. ovis<\/jats:italic>\n            -infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with\n            <jats:italic>B. ovis<\/jats:italic>\n            also reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type\n            <jats:italic>Brucella<\/jats:italic>\n            standard antigens.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.1.119-122.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"119-122","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["<i>Brucella abortus<\/i>\n            RB51 and Hot Saline Extract from\n            <i>Brucella ovis<\/i>\n            as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with\n            <i>Brucella abortus<\/i>\n            RB51"],"prefix":"10.1128","volume":"8","author":[{"given":"Rosanna","family":"Adone","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanita\u0300, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Ciuchini","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanita\u0300, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"149","article-title":"Uso della reazione di fissazione del complemento per il rilievo della risposta anticorpale in bovini vaccinati con il ceppo Brucella abortus RB51 in fase rugosa.","volume":"3","author":"Adone R.","year":"1999","unstructured":"Adone R. Ciuchini F. Uso della reazione di fissazione del complemento per il rilievo della risposta anticorpale in bovini vaccinati con il ceppo Brucella abortus RB51 in fase rugosa. Sel. Vet. 3 1999 149 155","journal-title":"Sel. Vet."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.6.787-790.1999"},{"key":"e_1_3_1_4_2","unstructured":"Alton\nG. G.\nJones\nL. M.\nAngus\nR. D.\nVerger\nJ. M.\nTechniques for the brucellosis laboratory.\n1988\nInstitut National de la Recherche Agronomique\nParis France"},{"key":"e_1_3_1_5_2","first-page":"12","article-title":"Brucella abortus strain RB51 vaccine: its advantages and risks.","volume":"213","author":"Karhel S. C.","year":"1998","unstructured":"Karhel S. C. Brucella abortus strain RB51 vaccine: its advantages and risks. Am. J. Vet. Med. Assoc. 213 1998 12 22","journal-title":"Am. J. Vet. Med. Assoc."},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879600800408"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879700900404"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.1998.0251"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90091-S"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.4.1065-1066.1994"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.8.1.119-122.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.8.1.119-122.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:10:56Z","timestamp":1645593056000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.8.1.119-122.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1128\/CDLI.8.1.119-122.2001"],"URL":"https:\/\/doi.org\/10.1128\/cdli.8.1.119-122.2001","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2000-04-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}