{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:40:50Z","timestamp":1792741250254,"version":"4.5.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An assay device for the rapid detection of\n            <jats:italic>Leptospira<\/jats:italic>\n            -specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.1.166-169.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"166-169","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":94,"title":["Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis"],"prefix":"10.1128","volume":"8","author":[{"given":"Henk L.","family":"Smits","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. K.","family":"Eapen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Malankara Orthodox Syrian Church Medical Mission Hospital, Kolenchery, India2;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Sugathan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Malankara Orthodox Syrian Church Medical Mission Hospital, Kolenchery, India2;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariamma","family":"Kuriakose","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Malankara Orthodox Syrian Church Medical Mission Hospital, Kolenchery, India2;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Hussein","family":"Gasem","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Medicine, Dr. Kariadi Hospital\/Diponegoro University, Semarang, Indonesia3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Yersin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Medicine, Victoria Hospital, The Seychelles4; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sasaki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Epidemiology Branch, Department of Health, Honolulu, Hawaii5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bambang","family":"Pujianto","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Medicine, Dr. Kariadi Hospital\/Diponegoro University, Semarang, Indonesia3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Vestering","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresia H.","family":"Abdoel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George C.","family":"Gussenhoven","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"237","article-title":"Leptospirosis worldwide, 1999.","volume":"74","author":"Anonymous","year":"1999","unstructured":"Anonymous Leptospirosis worldwide, 1999. 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