{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:07:26Z","timestamp":1791198446135,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Nucleic acid amplification was performed for five loci in the\n            <jats:italic>cag<\/jats:italic>\n            pathogenicity island (PAI) of\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            (comprising\n            <jats:italic>cagA<\/jats:italic>\n            , the\n            <jats:italic>cagA<\/jats:italic>\n            promoter region,\n            <jats:italic>cagE<\/jats:italic>\n            ,\n            <jats:italic>cagT<\/jats:italic>\n            , and the left end of\n            <jats:italic>cag<\/jats:italic>\n            II [LEC]), and gastric inflammation in patients was evaluated. Of 204\n            <jats:italic>H. pylori<\/jats:italic>\n            isolates from Japanese patients (53 with peptic ulcer, 55 with gastric cancer, and 96 with chronic gastritis), 197 (96.6%) were positive for all five loci. Two isolates (1%) were negative for all five loci, and five isolates (2.4%) were positive for only\n            <jats:italic>cagA<\/jats:italic>\n            and LEC. These latter seven isolates were all from patients with mild chronic gastritis. Neutrophil infiltration in gastric mucosa was significantly milder in patients infected with partially or totally deleted-PAI strains than in those with intact-PAI strains. The\n            <jats:italic>cagE<\/jats:italic>\n            gene was a more accurate marker of an intact\n            <jats:italic>cag<\/jats:italic>\n            PAI than the\n            <jats:italic>cagA<\/jats:italic>\n            gene, and\n            <jats:italic>cagE<\/jats:italic>\n            seemed to be more useful in discriminating between\n            <jats:italic>H. pylori<\/jats:italic>\n            strains causing different rates of disease progression.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.1.181-186.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"181-186","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":77,"title":["Determination of\n            <i>Helicobacter pylori<\/i>\n            Virulence by Simple Gene Analysis of the\n            <i>cag<\/i>\n            Pathogenicity Island"],"prefix":"10.1128","volume":"8","author":[{"given":"Tsuneo","family":"Ikenoue","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Maeda","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiji","family":"Ogura","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Akanuma","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzo","family":"Mitsuno","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Imai","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruhiko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Shiratori","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Omata","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00770.x"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.4.593"},{"key":"e_1_3_1_4_2","first-page":"2111","article-title":"Infection with Helicobacter pylori strains possessing cagA associated with an increased risk of developing adenocarcinoma of the stomach.","volume":"55","author":"Blaser M. 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