{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T01:59:53Z","timestamp":1789351193761,"version":"build-2803163510"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An enzyme-linked immunosorbent assay (ELISA) was adapted to measure immunoglobulin G (IgG), IgM, and IgA classes of human serum antibody to\n            <jats:italic>Campylobacter jejuni<\/jats:italic>\n            and\n            <jats:italic>Campylobacter coli<\/jats:italic>\n            . Heat-stable antigen, a combination of\n            <jats:italic>C. jejuni<\/jats:italic>\n            serotype O:1,44 and O:53 in the ratio 1:1, was used as a coating antigen in the ELISA test. A total of 631 sera from 210 patients with verified\n            <jats:italic>Campylobacter<\/jats:italic>\n            enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. With a 90th percentile of specificity, IgG, IgM, and IgA showed a sensitivity of 71, 60, and 80%, respectively. By combining all three antibody classes, the sensitivity was 92% within 35 days after infection, whereas within 90 days after infection, a combined sensitivity of 90% was found (IgG 68%, IgM 52%, and IgA 76%). At follow-up of the patients, IgG antibodies were elevated 4.5 months after infection but exhibited a large degree of variation in antibody decay profiles. IgA and IgM antibodies were elevated during the acute phase of infection (up to 2 months from onset of infection). The antibody response did not depend on\n            <jats:italic>Campylobacter<\/jats:italic>\n            species or\n            <jats:italic>C. jejuni<\/jats:italic>\n            serotype, with the important exception of response to\n            <jats:italic>C. jejuni<\/jats:italic>\n            O:19, the serotype most frequently associated with Guillain-Barre\u0301 syndrome. All of the patients infected with this serotype had higher levels of both IgM (\n            <jats:italic>P<\/jats:italic>\n            = 0.006) and IgA (\n            <jats:italic>P<\/jats:italic>\n            = 0.06) compared with other\n            <jats:italic>C. jejuni<\/jats:italic>\n            and\n            <jats:italic>C. coli<\/jats:italic>\n            serotypes.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.2.314-319.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"314-319","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Antibody Responses to\n            <i>Campylobacter<\/i>\n            Infections Determined by an Enzyme-Linked Immunosorbent Assay: 2-Year Follow-Up Study of 210 Patients"],"prefix":"10.1128","volume":"8","author":[{"given":"Mette Aagaard","family":"Strid","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastrointestinal Infections1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen","family":"Engberg","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastrointestinal Infections1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena Brandt","family":"Larsen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastrointestinal Infections1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamilla","family":"Begtrup","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Epidemiology Research,2 Statens Serum Institut, DK-2300 Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e5re","family":"M\u00f8lbak","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastrointestinal Infections1 and"},{"name":"<!--label omitted: 2-->Department of Epidemiology Research,2 Statens Serum Institut, DK-2300 Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Angeliki","family":"Krogfelt","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Gastrointestinal Infections1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0402.980213"},{"key":"e_1_3_2_3_2","unstructured":"Andersen\nL. P.\nGaarslev\nK. Campylobacter jejuni\/coli : elevated IgA and IgM antibodies during acute infection\nProceedings of the Fifth International Workshop on Campylobacter Infections.\nRuiz-Palacios\nG. M.\nCalva\nF.\nRuiz-Palacios\nB. R.\n1991\n221\n222\nInstituto Nacional de la Nutricion\nMexico DF Mexico"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.44.2.292-298.1984"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.5.820"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S095026880005024X"},{"key":"e_1_3_2_7_2","unstructured":"Campylobacter jejuni\/coli Br\u00f8ndsted\nT.\nHald\nT.\nJ\u00f8rgensen\nB. B.\nAnnual Report on Zoonoses in Denmark 1999.\n2000\n20\n23\nMinistry of Food Agriculture and Fisheries\nCopenhagen Denmark"},{"key":"e_1_3_2_8_2","unstructured":"Diggle\nP. J\nLiang\nK. Y.\nZeger\nS. L.\nAnalysis of longitudinal data.\n1994\nClarendon Press Oxford University\nOxford U.K"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.286-291.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.5.536-539.1997"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.1.1-4.1983"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410330304"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1993.tb00159.x"},{"key":"e_1_3_2_14_2","first-page":"51","article-title":"Detection of serum antibody response in patients infected with one strain of Campylobacter jejuni with a DIG-ELISA method.","volume":"9","author":"Melby K.","year":"1986","unstructured":"Melby K. T\u00f8njum T. Skj\u00f8rten F. Detection of serum antibody response in patients infected with one strain of Campylobacter jejuni with a DIG-ELISA method. NIPH (Natl. Inst. Public Health) Ann. 9 1986 51 60","journal-title":"NIPH (Natl. Inst. Public Health) Ann."},{"key":"e_1_3_2_15_2","unstructured":"Nachamkin\nI.\nEngberg\nJ.\nAastrup\nF. M.\nDiagnosis and susceptibility of Campylobacter spp.\nCampylobacter.\nNachamkin\nI.\nBlaser\nM. J.\n2000\n45\n68\nASM Press\nWashington D.C."},{"key":"e_1_3_2_16_2","unstructured":"Nachamkin\nI.\nMurray\nE. J.\nBaron\nM. A.\nPfaller\nF. C.\nTenover\nF.\nYolken\nR. H. Campylobacter and Arcobacter Manual of clinical microbiology\n6th ed.\nMurray\nP. R.\n1995\n483\n491\nASM Press\nWashington D.C."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/513789"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1997.tb01071.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02019474"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.10.1944-1951.1987"},{"key":"e_1_3_2_21_2","unstructured":"Sackett\nD. L.\nHaynes\nR. B.\nGuyatt\nG. H.\nTugwell\nP.\nClinical epidemiology: a basic science for clinical medicine\n1991\n69\n152\nLittle Brown & Co.\nBoston Mass"},{"key":"e_1_3_2_22_2","unstructured":"Skirrow\nM. B.\nBlaser\nM. J. Campylobacter jejuni Infections of the gastrointestinal tract.\nBlaser\nM. J.\nSmith\nP. D.\nRavdin\nJ. I.\nGreenberg\nH. B.\nGuerrant\nR. L.\n1995\n825\n848\nRaven Press\nNew York N.Y"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.1.82"},{"key":"e_1_3_2_24_2","first-page":"423","article-title":"Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni, and its clinical application.","volume":"90","author":"Walder M.","year":"1982","unstructured":"Walder M. Forsgren A. Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni, and its clinical application. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 90 1982 423 433","journal-title":"Acta Pathol. Microbiol. Immunol. Scand. Sect. 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