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None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate<jats:italic>T. pallidum<\/jats:italic>antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.<\/jats:p>","DOI":"10.1128\/cdli.8.3.534-539.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"534-539","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Western Immunoblotting with Five<i>Treponema pallidum<\/i>Recombinant Antigens for Serologic Diagnosis of Syphilis"],"prefix":"10.1128","volume":"8","author":[{"given":"Vittorio","family":"Sambri","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Section of Microbiology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Marangoni","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Section of Microbiology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Eyer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mikrogen GmbH, Martinsried, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Reichhuber","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mikrogen GmbH, Martinsried, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Soutschek","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Mikrogen GmbH, Martinsried, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Negosanti","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Section of Dermatology,3 DMCSS, University of Bologna, St. Orsola Hospital, Bologna, Italy, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonietta","family":"D'Antuono","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Section of Dermatology,3 DMCSS, University of Bologna, St. Orsola Hospital, Bologna, Italy, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Cevenini","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Section of Microbiology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"4363","DOI":"10.4049\/jimmunol.141.12.4363","article-title":"Development of cellular immunity to individual soluble antigen of Treponema pallidum during experimental syphilis.","volume":"141","author":"Baker-Zander S. 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