{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:28:08Z","timestamp":1760120888252},"reference-count":15,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previously a\n            <jats:italic>Brucella<\/jats:italic>\n            protein named CP28, BP26, or Omp28 has been identified as an immunodominant antigen in infected cattle, sheep, goats, and humans. In the present study we evaluated antibody responses of infected and\n            <jats:italic>B. melitensis<\/jats:italic>\n            Rev.1-vaccinated sheep to the BP26 protein using purified recombinant BP26 protein produced in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            in an indirect enzyme-linked immunosorbent assay (I-ELISA). The specificity of the I-ELISA determined with sera from healthy sheep (\n            <jats:italic>n<\/jats:italic>\n            = 106) was 93%. The sensitivity of the I-ELISA assessed with sera from naturally infected and suspected sheep found positive in the current conventional diagnostic tests was as follows: 100% for bacteriologically and serologically positive sheep (\n            <jats:italic>n<\/jats:italic>\n            = 50), 88% for bacteriologically negative but serologically and delayed-type hypersensitivity-positive sheep (\n            <jats:italic>n<\/jats:italic>\n            = 50), and 84% for bacteriologically and serologically negative but delayed-type hypersensitivity-positive sheep (\n            <jats:italic>n<\/jats:italic>\n            = 19). However, the absorbance values observed did not reach those observed in an I-ELISA using purified O-polysaccharide (O-PS) as an antigen. In sheep experimentally infected with\n            <jats:italic>B. melitensis<\/jats:italic>\n            H38 the antibody response to BP26 was delayed and much weaker than that to O-PS. Nevertheless, the BP26 protein appears to be a good diagnostic antigen to be used in confirmatory tests and for serological differentiation between infected and\n            <jats:italic>B. melitensis<\/jats:italic>\n            Rev.1-vaccinated sheep. Weak antibody responses to BP26 in some of the latter sheep suggest that a\n            <jats:italic>B. melitensis<\/jats:italic>\n            Rev.1\n            <jats:italic>bp26<\/jats:italic>\n            gene deletion mutant should be constructed to ensure this differentiation.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.4.772-775.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"772-775","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Use of Recombinant BP26 Protein in Serological Diagnosis of\n            <i>Brucella melitensis<\/i>\n            Infection in Sheep"],"prefix":"10.1128","volume":"8","author":[{"given":"Axel","family":"Cloeckaert","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Baucheron","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nieves","family":"Vizcaino","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Departmento Microbiolog\u0131\u0301a y Gene\u0301tica, Edificio Departamental, Universidad de Salamanca, 37007 Salamanca, Spain2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel S.","family":"Zygmunt","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(96)01110-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.2.798-800.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-45-3-206"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08327.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)00058-5"},{"key":"e_1_3_2_7_2","first-page":"255","article-title":"Brucella melitensis infection in sheep: present and future.","volume":"29","author":"Garin-Bastuji B.","year":"1998","unstructured":"Garin-Bastuji B. Blasco J. M. Grayon M. Verger J. M. Brucella melitensis infection in sheep: present and future. Vet. Res. 29 1998 255 274","journal-title":"Vet. 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