{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T09:05:37Z","timestamp":1759482337764},"reference-count":25,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Splenic-macrophage Fc\u03b3 receptors (Fc\u03b3Rs) participate in the pathophysiologies of immune-complex diseases and in host defense against infection. Modulation of macrophage Fc\u03b3R expression is an immuno-therapeutic target. Glucocorticoids, sex steroids, and dopaminergic drugs modulate macrophage Fc\u03b3R expression. Previous data indicate that estradiol increases macrophage Fc\u03b3R expression. Nevertheless, the effects of clinically used estrogens upon macrophage Fc\u03b3R expression are unknown. We assessed the effects of treatment with commonly used estrogens on the expression of macrophage Fc\u03b3Rs using a guinea pig experimental model. Six estrogens have been studied: ethynylestradiol (Et), mestranol (M), chlortianisene (Ct), promestriene, 17-epiestriol, and 17\u03b2-estradiol. Following in vivo treatment of guinea pigs, we determined the clearance of immunoglobulin G (IgG)-sensitized erythrocytes in vivo, the binding of IgG-sensitized erythrocytes by isolated splenic macrophages, and splenic-macrophage Fc\u03b3R cell surface expression. Estrogens enhance the clearance of IgG-sensitized erythrocytes by increasing splenic-macrophage Fc\u03b3R expression. Et, M, and Ct were more effective than the other estrogens. Flow cytometry and fluorescence microscopy with monoclonal antibodies demonstrated that estrogens increase the cell surface expression of Fc\u03b3R1 and -2 more than that of Fc\u03b3R2. These data indicate that treatment with commonly used estrogens enhances the clearance of IgG-sensitized cells by improving splenic-macrophage Fc\u03b3R expression.<\/jats:p>","DOI":"10.1128\/cdli.8.4.806-810.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"806-810","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Enhancement of Splenic-Macrophage Fc\u03b3 Receptor Expression by Treatment with Estrogens"],"prefix":"10.1128","volume":"8","author":[{"given":"F.","family":"Gomez","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Hospital Universitario de Puerto Real\/S.A.S. and Department of Medicine, School of Medicine, University of Cadiz, Cadiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Universitario de Puerto Real\/S.A.S. and Department of Medicine, School of Medicine, University of Cadiz, Cadiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Bernal","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Universitario de Puerto Real\/S.A.S. and Department of Medicine, School of Medicine, University of Cadiz, Cadiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Escobar","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Universitario de Puerto Real\/S.A.S. and Department of Medicine, School of Medicine, University of Cadiz, Cadiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Garcia-Egido","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Universitario de Puerto Real\/S.A.S. and Department of Medicine, School of Medicine, University of Cadiz, Cadiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J. 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