{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:46:55Z","timestamp":1790567215609,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            No recombinant protein is available for serodiagnosis of melioidosis. In this study, we report the cloning of the\n            <jats:italic>groEL<\/jats:italic>\n            gene, which encodes an immunogenic protein of\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            . Bidirectional DNA sequencing of\n            <jats:italic>groEL<\/jats:italic>\n            revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kDa. Basic Local Alignment Search Tool analysis showed that the putative protein encoded by\n            <jats:italic>groEL<\/jats:italic>\n            is homologous to the chaperonins encoded by the\n            <jats:italic>groEL<\/jats:italic>\n            genes of other bacteria. It has 98% amino acid identity with the GroEL of\n            <jats:italic>Burkholderia cepacia,<\/jats:italic>\n            98% amino acid identity with the GroEL of\n            <jats:italic>Burkholderia vietnamiensis,<\/jats:italic>\n            and 82% amino acid identity with the GroEL of\n            <jats:italic>Bordetella pertussis<\/jats:italic>\n            . Furthermore, it was observed that patients with melioidosis develop a strong antibody response against GroEL, suggesting that the recombinant protein and its monoclonal antibody may be useful for serodiagnosis in patients with melioidosis and that the protein may represent a good cell surface target for host humoral immunity. Further studies in these directions would be warranted.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.4.832-836.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:53Z","timestamp":1027764233000},"page":"832-836","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["<i>groEL<\/i>\n            Encodes a Highly Antigenic Protein in\n            <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1128","volume":"8","author":[{"given":"Patrick C. Y.","family":"Woo","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia K. L.","family":"Leung","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samson S. Y.","family":"Wong","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pak-Leung","family":"Ho","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwok-Yung","family":"Yuen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and"},{"name":"<!--label omitted: 2-->HKU-Pasteur Research Centre,2 Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ausubel\nF. M.\nBrent\nR.\nKingston\nR. E.\nMoore\nD. D.\nSeidman\nJ. 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