{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:26:08Z","timestamp":1764782768014},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The gene encoding the pneumococcal surface adhesin A (PsaA) protein has been identified in three different viridans group streptococcal species. Comparative studies of the<jats:italic>psaA<\/jats:italic>gene identified in different pneumococcal isolates by sequencing PCR products showed a high degree of conservation among these strains. PsaA is encoded by an open reading frame of 930 bp. The analysis of this fragment in<jats:italic>Streptococcus mitis, Streptococcus oralis<\/jats:italic>, and<jats:italic>Streptococcus anginosus<\/jats:italic>strains revealed a sequence identity of 95, 94, and 90%, respectively, to the corresponding open reading frame of the previously reported<jats:italic>Streptococcus pneumoniae<\/jats:italic>serotype 6B strain. Our results confirm that<jats:italic>psaA<\/jats:italic>is present and detectable in heterologous bacterial species. The possible implications of these results for the suitability and potential use of PsaA in the identification and diagnosis of pneumococcal diseases are discussed.<\/jats:p>","DOI":"10.1128\/cdli.8.5.895-898.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"895-898","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Identification of the<i>psaA<\/i>Gene, Coding for Pneumococcal Surface Adhesin A, in Viridans Group Streptococci other than<i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1128","volume":"8","author":[{"given":"Isabel","family":"Jado","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Referencia de Neumococos, Centro Nacional de Microbiolog\u0131\u0301a, Instituto de Salud Carlos III, Majadahonda E-28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asuncio\u0301n","family":"Fenoll","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Referencia de Neumococos, Centro Nacional de Microbiolog\u0131\u0301a, Instituto de Salud Carlos III, Majadahonda E-28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Casal","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Referencia de Neumococos, Centro Nacional de Microbiolog\u0131\u0301a, Instituto de Salud Carlos III, Majadahonda E-28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amalia","family":"Pe\u0301rez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de Referencia de Neumococos, Centro Nacional de Microbiolog\u0131\u0301a, Instituto de Salud Carlos III, Majadahonda E-28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.12.5255-5262.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.796-800.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1993.03510150060030"},{"key":"e_1_3_2_5_2","first-page":"107","article-title":"Mouse myeloma-spleen cell hybrids: enhanced hybridization frequencies and rapid screening procedures.","volume":"81","author":"Clafin L.","year":"1978","unstructured":"Clafin L. 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