{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T07:15:26Z","timestamp":1726211726876},"reference-count":38,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The diagnostic and analytical performance of the coupled-particle light-scattering assay in detecting anti-Ro\/SSA autoantibodies (the 60-kDa [Ro60] and the 52-kDa [Ro52] antibodies) and anti-La\/SSB autoantibodies was evaluated. The antigens were obtained by recombinant DNA procedures to include the most immunogenic epitopes for each protein by using a prokaryotic expression system. Serum samples from 151 patients with connective tissue diseases and 52 control subjects (including patients with viral infections, patients with Lyme disease, and healthy subjects) were studied. Sensitivities for detection of anti-Ro\/SSA and anti-La\/SSB were 88.2 and 95.2%, respectively; specificities were 97.6 and 98.1%, respectively. The intra-assay coefficient of variation (CV) ranged from 4.3 to 10.9% for anti-Ro\/SSA and from 2.8 to 12.5% for anti-La\/SSB; interassay CVs ranged from 6.5 to 13.2% and from 8.2 to 14.5%, respectively. Among the anti-Ro\/SSA-positive samples, Ro60 was recognized by 66% of the test sera and Ro52 was recognized by 95% of the test sera. Thirty-four percent of the Ro\/SSA-positive sera were reactive only with the Ro52 antigen, indicating that anti-Ro52 is the most common antibody specificity recognized by anti-Ro\/SSA autoantibodies. No differences were found between the prevalences of anti-Ro60 and anti-Ro52 in relation to systemic lupus erythematosus or Sjo\u0308gren's syndrome. The results of the present study indicate that this new immunoassay is an efficient diagnostic tool for the detection of anti-Ro\/SSA and anti-La\/SSB antibodies in patients with autoimmune disorders.<\/jats:p>","DOI":"10.1128\/cdli.8.5.922-925.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"922-925","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["New Coupled-Particle Light-Scattering Assay for Detection of Ro\/SSA (52 and 60 Kilodaltons) and La\/SSB Autoantibodies in Connective Tissue Diseases"],"prefix":"10.1128","volume":"8","author":[{"given":"Nicola","family":"Bizzaro","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio di Patologia Clinica, Ospedale Civile, S. Dona\u0300 di Piave,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Bonelli","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Diasorin srl, Saluggia,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elio","family":"Tonutti","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Istituto di Chimica Clinica, Azienda Ospedaliera S. Maria della Misericordia, Udine,3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato","family":"Tozzoli","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Laboratorio Analisi Chimico-cliniche e Microbiologia, Ospedale Civile, Latisana,4 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danilo","family":"Villalta","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Laboratorio di Microbiologia e Immunologia, Azienda Ospedaliera S. Maria degli Angeli, Pordenone,5 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1992.tb06874.x"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1999.0298"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330307"},{"key":"e_1_3_1_5_2","first-page":"480","article-title":"Sensitivity of commercial reagents for the detection of anti-ENA antibodies.","volume":"37","author":"Bizzaro N.","year":"1999","unstructured":"Bizzaro N. Bassetti D. Manoni F. Piazza A. Tozzoli R. Tonutti E. Sensitivity of commercial reagents for the detection of anti-ENA antibodies. Clin. Chem. Lab. Med. 37 1999 480","journal-title":"Clin. Chem. Lab. Med."},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00140-9"},{"key":"e_1_3_1_7_2","unstructured":"Bodner\nA. J.\nBritz\nJ. A.\nCopalis technology\nImmunoassay automation: an updated guide to systems.\nChan\nD. W.\n1996\n253\n275\nAcademic Press Inc.\nSan Diego Calif"},{"key":"e_1_3_1_8_2","first-page":"1838","article-title":"Anti-Ro(SSA) antibodies: clinical significance and biological relevance.","volume":"23","author":"Bouffard P.","year":"1996","unstructured":"Bouffard P. Laniel M. A. Boire G. Anti-Ro(SSA) antibodies: clinical significance and biological relevance. J. Rheum. 23 1996 1838 1841","journal-title":"J. Rheum."},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.4.406"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330502"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1992.tb00810.x"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.7.2115"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81321-2"},{"key":"e_1_3_1_14_2","first-page":"1","article-title":"The SS-A\/Ro antigen","volume":"4","author":"Chan E. K. L.","year":"1994","unstructured":"Chan E. K. L. Buyon J. P. The SS-A\/Ro antigen Manual of biological markers of disease van Venrooij W. J. Maini R. N. B4.1 1994 1 18 Kluwer Academic Publishers Dordrecht, The Netherlands","journal-title":"Manual of biological markers of disease"},{"key":"e_1_3_1_15_2","first-page":"507","article-title":"The Consensus Workshop for the Detection of Autoantibodies to Intracellular Antigens in Rheumatic Diseases: 1989\u20131992.","volume":"10","author":"Charles P. J.","year":"1992","unstructured":"Charles P. J. van Venrooij W. J. Maini R. N. the Consensus Finding Group for Autoantibodies The Consensus Workshop for the Detection of Autoantibodies to Intracellular Antigens in Rheumatic Diseases: 1989\u20131992. Clin. Exp. Rheumatol. 10 1992 507 511","journal-title":"Clin. Exp. Rheumatol."},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1994.1027"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1998.4560"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.24.9479"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-8411(89)90167-4"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.22.6337"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.3.436-443.2000"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860090304"},{"key":"e_1_3_1_23_2","doi-asserted-by":"crossref","unstructured":"Keech\nC. L.\nMcCluskey\nJ.\nGordon\nT. P.\nSS-B(La) autoantibodies\nAutoantibodies.\nPeter\nJ. B.\nShoenfeld\nY.\n1996\n789\n797\nElsevier Science BV\nAmsterdam The Netherlands","DOI":"10.1016\/B978-044482383-0\/50121-7"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/32.6.449"},{"key":"e_1_3_1_25_2","first-page":"205","article-title":"Multicenter validation of recombinant, natural and synthetic antigens used in a single multiparameter assay for the detection of specific anti-nuclear autoantibodies in connective tissue disorders.","volume":"17","author":"Meheus L.","year":"1999","unstructured":"Meheus L. van Venrooij W. J. Wiik A. Charles P. J. Tzioufas A. G. Meyer O. Multicenter validation of recombinant, natural and synthetic antigens used in a single multiparameter assay for the detection of specific anti-nuclear autoantibodies in connective tissue disorders. Clin. Exp. Rheumatol. 17 1999 205 214","journal-title":"Clin. Exp. Rheumatol."},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90362-Y"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1177\/000456329803500104"},{"key":"e_1_3_1_28_2","first-page":"112","article-title":"Antibodies to Ro(SSA) and the heterogeneity of systemic lupus erythematosus.","volume":"14","author":"Reichlin M.","year":"1987","unstructured":"Reichlin M. Harley J. B. Antibodies to Ro(SSA) and the heterogeneity of systemic lupus erythematosus. J. Rheumatol. 14 1987 112 117","journal-title":"J. Rheumatol."},{"key":"e_1_3_1_29_2","doi-asserted-by":"crossref","unstructured":"Reichlin\nM.\nScofield\nR. H.\nSS-A(Ro) autoantibodies\nAutoantibodies.\nPeter\nJ. B.\nShoenfeld\nY.\n1996\n783\n788\nElsevier Science BV\nAmsterdam The Netherlands","DOI":"10.1016\/B978-044482383-0\/50120-5"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.1996.186316.x"},{"key":"e_1_3_1_31_2","unstructured":"Sambrook\nJ.\nFritsch\nE. F.\nManiatis\nT.\nMolecular cloning: a laboratory manual\n2nd ed.\n1989\nCold Spring Harbor Laboratory Press\nCold Spring Harbor N.Y"},{"key":"e_1_3_1_32_2","first-page":"868","article-title":"Hypergammaglobulinemic purpura in systemic autoimmune rheumatic diseases: predictive value of anti-Ro(SSA) and anti-La(SSB) antibodies and treatment with indomethacin and hydroxychloroquine.","volume":"22","author":"Senecal J. L.","year":"1995","unstructured":"Senecal J. L. Chartier S. Rothfield N. Hypergammaglobulinemic purpura in systemic autoimmune rheumatic diseases: predictive value of anti-Ro(SSA) and anti-La(SSB) antibodies and treatment with indomethacin and hydroxychloroquine. J. Rheumatol. 22 1995 868 875","journal-title":"J. Rheumatol."},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1991.tb05780.x"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199904)42:3<455::AID-ANR10>3.0.CO;2-3"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1664"},{"key":"e_1_3_1_36_2","first-page":"1","article-title":"Clinical significance of autoantibodies to Ro\/SSA and La\/SSB","volume":"4","author":"Tzioufas A. G.","year":"1996","unstructured":"Tzioufas A. G. Moutsopoulos H. M. Clinical significance of autoantibodies to Ro\/SSA and La\/SSB Manual of biological markers of disease van Venrooij W. J. Maini R. N. C4.1 1996 1 14 Kluwer Academic Publishers Dordrecht, The Netherlands","journal-title":"Manual of biological markers of disease"},{"key":"e_1_3_1_37_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.55.2.116"},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-0172(95)80004-2"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.4.561"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.8.5.922-925.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.8.5.922-925.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:12:35Z","timestamp":1645593155000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.8.5.922-925.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,9]]}},"alternative-id":["10.1128\/CDLI.8.5.922-925.2001"],"URL":"https:\/\/doi.org\/10.1128\/cdli.8.5.922-925.2001","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,9]]},"assertion":[{"value":"2000-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-05-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}