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The cystatin capture ELISA was investigated for its usefulness for the serodiagnosis of human clonorchiasis. Cystatin bound specifically to cysteine proteinases in crude extracts of adult<jats:italic>C. sinensis<\/jats:italic>worms, and its binding capacity was not hindered competitively by the other proteinase inhibitors tested. The cystatin capture ELISA for clonorchiasis showed a higher degree of specificity than the conventional ELISA, which produced some cross-reactivities to sera from patients with cysticercosis, sparganosis, and opisthorchiasis. Immunoglobulin G antibodies to<jats:italic>C. sinensis<\/jats:italic>cysteine proteinases were produced in experimental rabbits at week 3, and their levels increased rapidly and remained at a plateau after 8 weeks of infection. Of the proteins from the<jats:italic>C. sinensis<\/jats:italic>crude extract captured with cystatin, seven proteins were reactive with the serum from patients with clonorchiasis. The cystatin capture ELISA is indicated to be a sensitive and highly specific immunodiagnostic assay for serodiagnosis of human clonorchiasis.<\/jats:p>","DOI":"10.1128\/cdli.8.6.1076-1080.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:55:30Z","timestamp":1027749330000},"page":"1076-1080","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein of<i>Clonorchis sinensis<\/i>"],"prefix":"10.1128","volume":"8","author":[{"given":"Tae Yun","family":"Kim","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-Yong","family":"Kang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun Hyo","family":"Park","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kom","family":"Sukontason","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Parasitology, Chiang Mai University Faculty of Medicine, Chiang Mai 50200, Thailand2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kabkaew","family":"Sukontason","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Parasitology, Chiang Mai University Faculty of Medicine, Chiang Mai 50200, Thailand2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Jong","family":"Hong","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47989-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.19195"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2110129"},{"key":"e_1_3_2_5_2","first-page":"1363","article-title":"The cystatins: a diverse superfamily of cysteine peptidase inhibitors.","volume":"45","author":"Barrett A. 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