{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:51:43Z","timestamp":1789937503451,"version":"4.0.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2001,11,1]],"date-time":"2001-11-01T00:00:00Z","timestamp":1004572800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Neurocysticercosis (NC), caused by the presence of\n            <jats:italic>Taenia solium<\/jats:italic>\n            metacestodes in tissues, is a severe parasitic infection of the central nervous system with universal distribution. To determine the efficiency of enzyme-linked immunosorbent assay (ELISA) and immunoblot with antigens of\n            <jats:italic>T. crassiceps<\/jats:italic>\n            vesicular fluid (Tcra) compared to standard techniques (indirect immunofluorescence test [IFT] and complement fixation test [CFT]) using\n            <jats:italic>T. solium<\/jats:italic>\n            cysticerci (Tso) for the serodiagnosis of NC, we studied serum samples from 24 patients with NC, 30 supposedly healthy individuals, 76 blood bank donors, 45 individuals with other non-NC parasitoses, and 97 samples from individuals screened for cysticercosis serology (SC). The sensitivity observed was 100% for ELISA-Tso and ELISA-Tcra, 91.7% for the IFT, and 87.5% for the CFT. The specificity was 90% for ELISA-Tso, 96.7% for ELISA-Tcra, 50% for IFT, and 63.3% for CFT. The efficiency was highest for ELISA-Tcra, followed by ELISA-Tso, IFT, and CFT. Of the 23 samples from SC group, which were reactive to ELISA-Tso and\/or ELISA-Tcra, only 3 were positive to immunblot-Tcra (specific peptides of 14- and 18-kDa) and to glycoprotein peptides purified from Tcra antigen (gp-Tcra), showing the low predictive value of ELISA for screening. None of the samples from the remaining groups showed specific reactivity in immunoblot-Tcra. These results demonstrate that ELISA-Tcra can be used as a screening method for the serodiagnosis of NC and support the need for specific tests for confirmation of the results. The immunoblot can be used as a confirmatory test both with Tcra and gp-Tcra, with the latter having an advantage in terms of visualization of the results.\n          <\/jats:p>","DOI":"10.1128\/cdli.8.6.1140-1144.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"1140-1144","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Serodiagnosis of Human Cysticercosis by Using Antigens from Vesicular Fluid of\n            <i>Taenia crassiceps<\/i>\n            Cysticerci"],"prefix":"10.1128","volume":"8","author":[{"given":"Edne\u0301ia C.","family":"Bueno","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Clinical Immunology, Faculty of Pharmacy, University of the Vale do Itaja\u0131\u0301, Itaja\u0131\u0301SC,1 and"},{"name":"<!--label omitted: 2--> Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, University of Sa\u0303o Paulo,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Snege","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Fleury Laboratory,3 Sa\u0303o Paulo SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adelaide J.","family":"Vaz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Clinical Immunology, Faculty of Pharmacy, University of the Vale do Itaja\u0131\u0301, Itaja\u0131\u0301SC,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo G.","family":"Leser","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Fleury Laboratory,3 Sa\u0303o Paulo SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651996000300008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.146-151.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.24.4.536-541.1986"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.52.2.254"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651997000100006"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1139\/z62-086"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3324-3325.1995"},{"key":"e_1_3_2_9_2","first-page":"299","article-title":"Species-specific immunodiagnosis of Taenia solium cysticercosis by ELISA and immunoblotting.","volume":"38","author":"Gottstein B.","year":"1987","unstructured":"Gottstein B. Zini D. Schantz P. M. Species-specific immunodiagnosis of Taenia solium cysticercosis by ELISA and immunoblotting. Trop. Med. Parasitol. 38 1987 299 303","journal-title":"Trop. Med. Parasitol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00004-3"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2000)086[1001:TSMCAS]2.0.CO;2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2307\/3281534"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(89)80113-6"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90239-6"},{"key":"e_1_3_2_16_2","first-page":"926","article-title":"Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium.","volume":"114","author":"Larralde C.","year":"1990","unstructured":"Larralde C. Sotelo J. Montoya R. M. Palencia G. Padilla A. Govezensky T. Diaz M. L. Sciutto E. Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium. Arch. Pathol. Lab. Med. 114 1990 926 928","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_2_17_2","first-page":"119","article-title":"Apport de l'enzyme-linked immunoelectrotransfer blot assay au diagnostic de la neurocysticercose humaine.","volume":"38","author":"Michault A.","year":"1990","unstructured":"Michault A. Rivi\u00e8re B. Fressy P. Laporte J. P. Bertil G. Mignard C. Apport de l'enzyme-linked immunoelectrotransfer blot assay au diagnostic de la neurocysticercose humaine. Pathol. Biol. 38 1990 119 125","journal-title":"Pathol. Biol."},{"key":"e_1_3_2_18_2","first-page":"115","article-title":"Le serodiagnostic de la cysticercose par ELISA et Western blot. Son int\u00e9r\u00eat et ses limites \u00e0 Madagascar.","volume":"57","author":"Michel P.","year":"1990","unstructured":"Michel P. Michault A. Gruel J. C. Coulanges P. Le serodiagnostic de la cysticercose par ELISA et Western blot. Son int\u00e9r\u00eat et ses limites \u00e0 Madagascar. Arch. Inst. Pasteur Madagascar 57 1990 115 142","journal-title":"Arch. Inst. Pasteur Madagascar"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(88)90020-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.56.127"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199209033271004"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X1997000300001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X1993000100003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.1.50"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0888-0786(96)01071-2"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.57.354"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.5.1007"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.8.6.1140-1144.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.8.6.1140-1144.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:12:59Z","timestamp":1645593179000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.8.6.1140-1144.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["10.1128\/CDLI.8.6.1140-1144.2001"],"URL":"https:\/\/doi.org\/10.1128\/cdli.8.6.1140-1144.2001","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11]]},"assertion":[{"value":"2001-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-08-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}