{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:25:53Z","timestamp":1791199553391,"version":"4.2.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Recent isolation of<jats:italic>Tropheryma whipplei<\/jats:italic>(formerly<jats:italic>Trophyrema whippelii<\/jats:italic>), the agent of Whipple\u2019s disease, from the cardiac valve of a patient with Whipple\u2019s disease endocarditis now allows the detection of reactive epitopes that could be used in a serological assay. In order to propose an enzyme-linked immunosorbent assay (ELISA) that uses recombinant<jats:italic>T. whipplei<\/jats:italic>antigen, we first determined by Western blotting of human, mouse, and rabbit antisera that the common immunodominant epitope is an 84-kDa protein. We then produced 13 monoclonal antibodies (MAbs) against<jats:italic>T. whipplei<\/jats:italic>, 12 of which recognize this immunodominant epitope. These MAbs did not react with phylogenetically closely related bacteria or bacteria previously shown to be cross-reactive with<jats:italic>T. whipplei<\/jats:italic>, but they did react with two other strains of<jats:italic>T. whipplei<\/jats:italic>isolated, one from an ocular sample and the other from a duodenal biopsy specimen. By confocal microscopy, the MAbs allowed detection of<jats:italic>T. whipplei<\/jats:italic>within infected fibroblasts. The identification of the 84-kDa antigen with our MAbs will make it possible to develop a diagnostic antigen for use in a diagnostic ELISA for Whipple\u2019s disease.<\/jats:p>","DOI":"10.1128\/cdli.9.1.156-159.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:30Z","timestamp":1027763730000},"page":"156-159","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Monoclonal Antibodies to Immunodominant Epitope of<i>Tropheryma whipplei<\/i>"],"prefix":"10.1128","volume":"9","author":[{"given":"Zhongxing","family":"Liang","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, Faculte\u0301 de Me\u0301decine de Marseille, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"La Scola","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, Faculte\u0301 de Me\u0301decine de Marseille, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, Faculte\u0301 de Me\u0301decine de Marseille, 13385 Marseille Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3181\/00379727-72-17388","volume":"72","year":"1949","unstructured":"Black-Schaffer, B. 1949. The tinctoral demonstration of a glycoprotein in Whipple\u2019s disease. Proc. Soc. Exp. Biol. Med.72:225\u2013227.","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s150100070039","volume":"28","year":"2000","unstructured":"Dutly, F. 2000. Tropheryma whippelii DNA in saliva of patients without Whipple\u2019s disease. Infection28:219\u2013222.","journal-title":"Infection"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1016\/S0140-6736(99)01776-6","volume":"353","year":"1999","unstructured":"Ehrbar, H. U., P. Bauerfeind, F. Dutly, H. R. Koelz, and M. Altwegg. 1999. PCR-positive tests for Tropheryma whippelii in patients without Whipple\u2019s disease. Lancet353:2214.","journal-title":"Lancet"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1136\/jcp.38.4.372","volume":"38","year":"1985","unstructured":"Evans, D., and M. H. Ali. 1985. 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