{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:43:39Z","timestamp":1773287019190,"version":"3.50.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The complete coding cDNA of HSP60 from\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            was overexpressed in an\n            <jats:italic>Escherichia coli<\/jats:italic>\n            host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysis with the purified HSP60 recombinant protein. Immunoblots indicated that the recombinant\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            HSP60 was recognized by antibodies in 72 of 75 sera from paracoccidioidomycosis patients. No cross-reactivity was detected with individual sera from patients with aspergillosis, sporotrichosis, cryptococcosis, and tuberculosis. Reactivity to HSP60 was observed in sera from 9.52% of control healthy individuals and 11.5% of patients with histoplasmosis. The high sensitivity and specificity (97.3 and 92.5%, respectively) for HSP60 suggested that the recombinant protein can be used singly or in association with other recombinant antigens to detect antibody responses in\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            -infected patients.\n          <\/jats:p>","DOI":"10.1128\/cdli.9.2.374-377.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"374-377","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Heterologous Expression, Purification, and Immunological Reactivity of a Recombinant HSP60 from\n            <i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1128","volume":"9","author":[{"given":"Daniela A.","family":"Cunha","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Biologia Molecular, ICB, Universidade Federal de Goia\u0301s, 74001-970 Goia\u0302nia, Goia\u0301s"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roseli M.","family":"Zancope\u0301-Oliveira","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Micologia Me\u0301dica, Hospital Evandro Chagas, Fundac\u0327a\u0303o Oswaldo Cruz, Rio de Janeiro"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sueli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Felipe","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Molecular, Instituto de Biologia, Universidade de Brasi\u0301lia, Brasi\u0301lia D.F., Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia M.","family":"Salem-Izacc","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Molecular, ICB, Universidade Federal de Goia\u0301s, 74001-970 Goia\u0302nia, Goia\u0301s"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"George S.","family":"Deepe","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0560"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ce\u0301lia M. 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