{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:25:05Z","timestamp":1790036705335,"version":"4.0.1"},"reference-count":11,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Reverse transcriptase PCR (RT-PCR) for diagnosis of Rift Valley fever (RVF) was evaluated by using 293 human and animal sera sampled during an RVF outbreak in Mauritania in 1998. Results of the RT-PCR diagnostic method were compared with those of virus isolation (VI) and detection of immunoglobulin M (IgM) antibodies. Our results showed that RT-PCR is a specific, sensitive tool for RVF diagnosis in the early phase of the disease and that its results do not differ significantly from those obtained by VI. Moreover, the combined results of RT-PCR and IgM antibody detection were in 100% concordance with the results of VI.<\/jats:p>","DOI":"10.1128\/cdli.9.3.713-715.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:09:29Z","timestamp":1027750169000},"page":"713-715","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Use of Reverse Transcriptase PCR in Early Diagnosis of Rift Valley Fever"],"prefix":"10.1128","volume":"9","author":[{"given":"A. 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K.","family":"Se\u0300ne","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Diagne","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Sylla","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mondo","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Girault","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Marrama","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Spiegel","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Diallo","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bouloy","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Mathiot","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, Senegal,"},{"name":"Institut Pasteur, Groupe des bunyaviride\u0301s, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0166-0934(97)00182-1","volume":"70","year":"1998","unstructured":"Burt, F. J., P. A. Leman, J. F. Smith, and R. Swanepoel. 1998. The use of reverse transcription-polymerase chain reaction for the detection of viral nucleic acid in the diagnosis of Crimean Congo haemorrhagic fever. J. Virol. Methods70:129-137.","journal-title":"J. Virol. Methods"},{"key":"e_1_3_2_3_2","first-page":"905","volume":"9","year":"2000","unstructured":"Centers for Disease Control and Prevention. 2000. Outbreak of Rift Valley Fever\u2014Saudi Arabia, August-October, 2000. Morb. Mortal. Wkly. Rep.9:905-908.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/jmv.1890400211","volume":"40","year":"1993","unstructured":"Chungue, E., C. Roche, M. F. Lefevre, P. Barbazan, and S. Chanteau. 1993. Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction. J. Med. 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