{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T20:12:30Z","timestamp":1774296750247,"version":"3.50.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The humoral immune response against\n            <jats:italic>Leishmania braziliensis<\/jats:italic>\n            histone H1 by patients with cutaneous leishmaniasis is described. For this purpose, the protein was purified as a recombinant protein in a prokaryotic expression system and was assayed by enzyme-linked immunosorbent assay (ELISA) with a collection of sera from patients with cutaneous leishmaniasis and Chagas' disease. The assays showed that\n            <jats:italic>L. braziliensis<\/jats:italic>\n            histone H1 was recognized by 66% of the serum samples from patients with leishmaniasis and by 40% of the serum samples from patients with Chagas' disease, indicating that it acts as an immunogen during cutaneous leishmaniasis. In order to locate the linear antigenic determinants of this protein, a collection of synthetic peptides covering the\n            <jats:italic>L. braziliensis<\/jats:italic>\n            histone H1sequence was tested by ELISA. The experiments showed that the main antigenic determinant is located in the central region of this protein. Our results show that the recombinant\n            <jats:italic>L. braziliensis<\/jats:italic>\n            histone H1 is recognized by a significant percentage of serum samples from patients with cutaneous leishmaniasis, but use of this protein as a tool for the diagnosis of cutaneous leishmaniasis is hampered by the cross-reaction with sera from patients with Chagas' disease.\n          <\/jats:p>","DOI":"10.1128\/cdli.9.4.808-811.2002","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:07:23Z","timestamp":1027897643000},"page":"808-811","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Antigenicity of\n            <i>Leishmania braziliensis<\/i>\n            Histone H1 during Cutaneous Leishmaniasis: Localization of Antigenic Determinants"],"prefix":"10.1128","volume":"9","author":[{"given":"Emma","family":"Carmelo","sequence":"first","affiliation":[{"name":"Departamento de Parasitologi\u0301a, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S\/C de Tenerife, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Marti\u0301nez","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologi\u0301a, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S\/C de Tenerife, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Cristina","family":"Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologi\u0301a, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S\/C de Tenerife, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301 Enrique","family":"Pin\u0303ero","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologi\u0301a, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S\/C de Tenerife, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel E.","family":"Patarroyo","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a San Juan de Dios, Bogota\u0301, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"del Castillo","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologi\u0301a, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S\/C de Tenerife, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basilio","family":"Valladares","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologi\u0301a, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S\/C de Tenerife, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0166-6851(94)90082-5","volume":"65","year":"1994","unstructured":"Aslund, L., L. Carlsson, J. Henriksson, M. Rydoiker, G. C. Toro, N. Galanti, and U. Petterson. 1994. A gene family encoding heterogeneous histone H1 proteins in Trypanosoma cruzi. Mol. Biochem. Parasitol.65:317-330.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.2.318-321.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1172\/JCI117305","volume":"94","year":"1994","unstructured":"Burlingame, R. W., M. L. Boey, G. Starkebaum, and R. L. Rubin. 1994. The central role of chromatin in autoimmune reponses to histones and DNA in systemic lupus erythematosus. J. Clin. Investig.94:184-192.","journal-title":"J. Clin. Investig."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/BF00932507","volume":"79","year":"1993","unstructured":"Burri, M., W. Schlimme, B. Betschart, U. Kampfer, J. Schaller, and H. Hecker. 1993. Biochemical and functional characterization of histone H1-like proteins in procyclic Trypanosoma brucei brucei. Parasitol. Res.79:649-659.","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1365-3024.1995.tb00881.x","volume":"17","year":"1995","unstructured":"Cerde\u00f1osa, N., C. Riera, P. Cort\u00e9s, F. March, C. Mu\u00f1oz, M. Port\u00fas, and G. Prats. 1995. Detection and characterization by immunoblot analysis of potentially diagnostic Leishmania infantum polypeptides in human visceral leishmaniasis. Parasite Immunol.17:509-516.","journal-title":"Parasite Immunol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1172\/JCI118969","volume":"98","year":"1996","unstructured":"Cunha-Neto, E., V. Coelho, L. Guilherme, A. Fiorelli, L. Stolf, and J. Kalil. 1996. Autoimmunity in Chagas' disease\u2014identification of cardiac myosin-B13 Trypanosoma cruzi protein crossreactive T-cell clones in heart lesions of a chronic Chagas' disease cardiomyopathy patient. J. Clin. Investig.98:1709-1712.","journal-title":"J. Clin. Investig."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0167-4838(90)90071-M","volume":"1040","year":"1990","unstructured":"Duschak, V. G., and J. J. Cazzulo. 1990. The histones of the insect trypanosomatid Chritidia fasciculata. Biochim. Biophys. Acta1040:159-166.","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/excr.1996.0105","volume":"224","year":"1996","unstructured":"Espinoza, I., G. C. Toro, V. Hellman, and N. Galanti. 1996. Histone H1 and core histones in Leishmania and Crithidia: comparison with Trypanosoma. Exp. Cell Res.224:1-7.","journal-title":"Exp. Cell Res."},{"key":"e_1_3_2_10_2","first-page":"321","volume":"62","year":"1994","unstructured":"Fasel, N. J., D. C. Robyr, J. Mauel, and T. A. Glaser. 1994. Identification of a histone H1-like gene expressed in Leishmania major. Mol. Biochem. Parasitol.62:321-324.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"5131","DOI":"10.1073\/pnas.82.15.5131","volume":"82","year":"1985","unstructured":"Houghten, R. A. 1985. General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids. Proc. Natl. Acad. Sci. USA82:5131-5135.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.210"},{"key":"e_1_3_2_13_2","first-page":"680","volume":"27","year":"1970","unstructured":"Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature27:680-685.","journal-title":"Nature"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1645\/0022-3395(2002)088[0199:CAMCOT]2.0.CO;2","volume":"88","year":"2002","unstructured":"Mart\u00ednez, E., M. C. Thomas, V. Alonso, E. Carmelo, A. C. Gonz\u00e1lez, A. Del Castillo, and B. Valladares. 2002. Cloning and molecular characterization of the DNA encoding histone H1 from Leishmania braziliensis. J. Parasitol.88:199-203.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1159\/000234962","volume":"89","year":"1989","unstructured":"Muller, S., D. Bonnier, M. Thiry, and M. H. V. Van Regenmortel. 1989. Reactivity to autoantibodies in systemic lupus erythematosus with synthetic core histone peptides. Int. Arch. Allergy Appl. Immunol.89:288-296.","journal-title":"Int. Arch. Allergy Appl. Immunol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/S0169-4758(00)01651-3","volume":"16","year":"2000","unstructured":"Requena, J. M., C. Alonso, and M. Soto. 2000. Evolutionarily conserved proteins as prominent antigens during Leishmania infections. Parasitol. Today16:246-249.","journal-title":"Parasitol. Today"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0165-2478(95)02473-5","volume":"48","year":"1995","unstructured":"Soto, M., J. M. Requena, L. Quijada, M. Garc\u00eda, F. Guzm\u00e1n, M. E. Patarroyo, and C. Alonso. 1995. Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis. Immunol. Lett.48:209-214.","journal-title":"Immunol. Lett."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1046\/j.1365-2249.1996.d01-865.x","volume":"106","year":"1996","unstructured":"Soto, M., J. M. Requena, L. Quijada, L. C. G\u00f3mez, F. Guzm\u00e1n, M. E. Patarroyo, and C. Alonso. 1996. Characterization of the antigenic determinants of the Leishmania infantum histone H3 recognized by antibodies elicited during canine visceral leishmaniasis. Clin. Exp. Immunol.106:454-461.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1046\/j.1365-2249.1999.00796.x","volume":"115","year":"1999","unstructured":"Soto, M., J. M. Requena, L. Quijada, M. J. Perez, C. G. Nieto, F. Guzman, M. E. Patarroyo, and C. Alonso. 1999. Antigenicity of the Leishmania infantum histones H2B and H4 during canine viscerocutaneous leishmaniasis. Clin. Exp. Immunol.115:342-349.","journal-title":"Clin. Exp. Immunol."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.9.4.808-811.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.9.4.808-811.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:32:13Z","timestamp":1627590733000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.9.4.808-811.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,7]]}},"alternative-id":["10.1128\/CDLI.9.4.808-811.2002"],"URL":"https:\/\/doi.org\/10.1128\/cdli.9.4.808-811.2002","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,7]]},"assertion":[{"value":"2001-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}