{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T15:10:45Z","timestamp":1779894645811,"version":"3.53.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Measurement of cellular immunity in human coccidioidomycosis has important diagnostic and prognostic implications. The coccidioidin skin test has been the standard for the measurement of this, but it is not available in the United States. We examined the utility of measuring surface expression of CD69 on T lymphocytes in whole blood incubated with the coccidioidal antigen preparation T27K as an alternative to the skin test. Seventy donors with active coccidioidomycosis were studied. The mean fluorescent intensity (MFI) of CD69 expression on CD3 lymphocytes in response to T27K was 28.61 \u00b1 1.77, significantly greater than the control response of 11.45 \u00b1 0.78 (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). The MFI CD69 response to T27K above that for the control (MFI CD69 above control) was 6.35 \u00b1 2.18 for seven subjects with disseminated coccidioidomycosis who were studied within 5 months of diagnosis. This was significantly below the value of 20.17 \u00b1 3.17 for 18 subjects with pulmonary coccidioidomycosis studied within 5 months of diagnosis and the value of 19.58 \u00b1 2.91 for 27 subjects with disseminated coccidioidomycosis studied after 5 months of diagnosis (for both,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). There was an inverse correlation between coccidioidal clinical score and MFI CD69 above control for all 34 subjects with disseminated coccidioidomycosis (\n            <jats:italic>r<\/jats:italic>\n            = 0.362;\n            <jats:italic>P<\/jats:italic>\n            = 0.036) but not for the 36 subjects with pulmonary disease (\n            <jats:italic>r<\/jats:italic>\n            &lt; 0.001;\n            <jats:italic>P<\/jats:italic>\n            = 0.993). Among 30 subjects for whom data were available, there was a highly significant association between the MFI CD69 above control and the supernatant concentrations of gamma interferon, interleukin-2 (IL-2), and tumor necrosis factor alpha (for all,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001), but not for IL-4, IL-5, or IL-10. These data indicate that in vitro assessment of CD69 expression on T lymphocytes by using T27K may be a useful measure of cellular immune response among subjects with active coccidioidomycosis.\n          <\/jats:p>","DOI":"10.1128\/cdli.9.5.1039-1043.2002","type":"journal-article","created":{"date-parts":[[2002,8,30]],"date-time":"2002-08-30T18:44:29Z","timestamp":1030733069000},"page":"1039-1043","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["In Vitro Whole-Blood Analysis of Cellular Immunity in Patients with Active Coccidioidomycosis by Using the Antigen Preparation T27K"],"prefix":"10.1128","volume":"9","author":[{"given":"Neil M.","family":"Ampel","sequence":"first","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa A.","family":"Kramer","sequence":"additional","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijin","family":"Li","sequence":"additional","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah S.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen M.","family":"Kerekes","sequence":"additional","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demosthenes","family":"Pappagianis","sequence":"additional","affiliation":[{"name":"Medicine and Primary Care Service"},{"name":"Research Service, Southern Arizona Veterans Affairs Health Care System"},{"name":"Department of Medicine"},{"name":"Department of Microbiology and Immunology, University of Arizona, Tucson, Arizona"},{"name":"Department of Microbiology and Immunology of the University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1093\/infdis\/165.4.710","volume":"165","year":"1992","unstructured":"Ampel, N. M., G. C. Bejarano, S. D. Salas, and J. N. Galgiani. 1992. In vitro assessment of cellular immunity in human coccidioidomycosis: relationship between dermal hypersensitivity, lymphocyte transformation, and lymphokine production by peripheral blood mononuclear cells from healthy adults. J. Infect. Dis.165:710-715.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.11.4483-4487.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/mmy.39.4.315.320","volume":"39","year":"2001","unstructured":"Ampel, N. M., L. A. Kramer, K. M. Kerekes, S. M. Johnson, and D. Pappagianis. 2001. Assessment of the human immune response to T27K, a coccidioidal antigen preparation, by flow cytometry of whole blood. Med. Mycol.39:315-320.","journal-title":"Med. Mycol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1086\/515044","volume":"27","year":"1998","unstructured":"Ampel, N. 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