{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:38:03Z","timestamp":1787161083986,"version":"build-2736575974"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    gp43 is the major diagnostic antigen of\n                    <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n                    , the agent of paracoccidioidomycosis (PCM) in humans. In the present study, cDNA of the gp43 gene (\n                    <jats:italic>PbGP43<\/jats:italic>\n                    ) was obtained by reverse transcriptase PCR, inserted into a pGEX vector in frame with the glutathione\n                    <jats:italic>S<\/jats:italic>\n                    -transferase (GST) gene, and expressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    as inclusion bodies. Immunoblotting showed that all sera from patients with chronic pulmonary and acute lymphatic forms of PCM reacted with the recombinant fusion protein of the mature gp43 (381 amino acids). Reactivity with fusion proteins containing subfragments of the N-terminal, internal, or C-terminal regions occurred eventually, and the C-terminal region was the most antigenic. Lack of reactivity with the subfragments may be due to the conformational nature of the gp43 epitopes. Sera from patients with aspergillosis, candidiasis, and histoplasmosis did not react with the gp43-GST fusion protein. Our results suggest that recombinant gp43 corresponding to the processed antigen can be a useful tool in the diagnosis of PCM.\n                  <\/jats:p>","DOI":"10.1128\/cdli.9.6.1200-1204.2002","type":"journal-article","created":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T14:04:24Z","timestamp":1036159464000},"page":"1200-1204","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Expression in Bacteria of the Gene Encoding the gp43 Antigen of\n                    <i>Paracoccidioides brasiliensis<\/i>\n                    : Immunological Reactivity of the Recombinant Fusion Proteins"],"prefix":"10.1128","volume":"9","author":[{"given":"Susana N.","family":"Diniz","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka\u0301tia C.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patri\u0301cia S.","family":"Cisalpino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 F.","family":"Silveira","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R.","family":"Travassos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana","family":"Puccia","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1093\/glycob\/6.5.507","volume":"6","year":"1996","unstructured":"Almeida, I. C., D. C. A. Neville, A. Mehlert, A. Treumann, M. A. J. Ferguson, J. O. Previato, and L. R. Travassos. 1996. Structure of the N-linked oligosaccharides of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis. Glycobiology6:507-515.","journal-title":"Glycobiology"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/jcla.1860080614","volume":"8","year":"1994","unstructured":"Almeida, I. C., E. G. Rodrigues, and L. R. Travassos. 1994. Chemiluminescent immunoassays: discrimination between the reactivities of natural and human patient antibodies with antigens of eukaryotic pathogens, Trypanosoma cruzi and Paracoccidioides brasiliensis. J. Clin. Lab. Anal.8:424-431.","journal-title":"J. Clin. Lab. Anal."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.3.671-676.1993"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/02681219780001161","volume":"35","year":"1997","unstructured":"Bueno, J. P., M. J. Mendes Giannini, G. M. Del Negro, C. M. Assis, C. K. Takiguti, and M. A. Shikanai Yasuda. 1997. IgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: comparison of counterimmunoelectrophoresis and complement fixation. J. Med. Vet. Mycol.35:213-217.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.10.2377-2381.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"4553","DOI":"10.1074\/jbc.271.8.4553","volume":"271","year":"1996","unstructured":"Cisalpino, P. S., R. Puccia, L. M. Yamauchi, M. I. N. Cano, J. F. Silveira, and L. R. Travassos. 1996. Cloning, characterization, and epitope expression of the major diagnostic antigen of Paracoccidioides brasiliensis. J. Biol. Chem.271:4553-4560.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_8_2","first-page":"461","year":"1994","unstructured":"Cisalpino, P. S., J. F. Silveira, and L. R. Travassos. 1994. RNA and DNA isolation from Paracoccidioides brasiliensis yeast cells: establishment of cDNA and genomic libraries, and PCR amplification, p. 461-467. In B. Maresca and G. S. Kobayashi (ed.), Molecular biology of pathogenic fungi, a laboratory manual. Telos Press, New York, N.Y.","journal-title":"Molecular biology of pathogenic fungi, a laboratory manual"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/02681218780000021","volume":"25","year":"1987","unstructured":"Franco, M. 1987. Host-parasite relationships in paracoccidioidomycosis. J. Med. Vet. Mycol.25:5-18.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1089\/hyb.1996.15.415","volume":"15","year":"1996","unstructured":"Gesztesi, J. L., R. Puccia, L. R. Travassos, A. P. Vicentini, M. F. Franco, and J. D. Lopes. 1996. Monoclonal antibodies against the 43,000 Da glycoprotein from Paracoccidioides brasiliensis modulate laminin-mediated fungal adhesion to epithelial cells and pathogenesis. Hybridoma15:415-422.","journal-title":"Hybridoma"},{"key":"e_1_3_2_11_2","first-page":"680","volume":"224","year":"1970","unstructured":"Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature224:680-685.","journal-title":"Nature"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.4269\/ajtmh.1990.43.200","volume":"43","year":"1990","unstructured":"Mendes-Giannini, M. J. S., J. P. Bueno, M. A. Shikanai-Yasuda, A. M. S. Stolf, A. Masuda, V. Amato Neto, and A. W. Ferreira. 1990. Antibody response to the 43 kDa glycoprotein of Paracoccidioides brasiliensis as a marker for the evaluation of patients under treatment. Am. J. Trop. Med. Hyg.43:200-206.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.3960-3966.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1080\/02681219580000461","volume":"33","year":"1995","unstructured":"Moura-Campos, C., J. L. Gesztesi, A. P. Vincentini, J. D. Lopes, and Z. P. Camargo. 1995. Expression and isoforms of gp43 in different strains of Paracoccidioides brasiliensis. J. Med. Vet. Mycol.33:223-227.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1016\/S0264-410X(00)00074-8","volume":"18","year":"2000","unstructured":"Pinto, A. R., R. Puccia, S. N. Diniz, M. F. Franco, and L. R. Travassos. 2000. DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensis. Vaccine18:3050-3058.","journal-title":"Vaccine"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.8.1610-1615.1991"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/0003-9861(91)90475-X","volume":"289","year":"1991","unstructured":"Puccia, R., and L. R. Travassos. 1991. The 43-kDa glycoprotein from Paracoccidioides brasiliensis and its deglycosylated form: excretion and susceptibility to proteolysis. Arch. Biochem. Biophys.289:298-302.","journal-title":"Arch. Biochem. Biophys."},{"key":"e_1_3_2_18_2","first-page":"193","volume":"53","year":"1986","unstructured":"Puccia, R., S. Schenkman, P. A. J. Gorin, and L. R. Travassos. 1986. Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen. Infect. Immun.53:193-203.","journal-title":"Infect. Immun."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1080\/02681219180000101","volume":"29","year":"1991","unstructured":"Puccia, R., D. T. Takaoka, and L. R. Travassos. 1991. Purification of the 43 kDa glycoprotein from exocellular components excreted by Paracoccidioides brasiliensis in liquid culture (TOM medium). J. Med. Vet. Mycol.29:57-60.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1080\/00362177885380211","volume":"16","year":"1978","unstructured":"Restrepo, A., M. Restrepo, F. Restrepo, L. H. Aristiz\u00e1bal, L. H. Moncada, and H. Velez. 1978. Immune responses in paracoccidioidomycosis. A controlled study of 16 patients before and after treatment. Sabouraudia16:151-163.","journal-title":"Sabouraudia"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1080\/02681219480000111","volume":"32","year":"1994","unstructured":"Rodrigues, E. G., and L. R. Travassos. 1994. Nature of the reactive epitopes in Paracoccidioides brasiliensis polysaccharide antigen. J. Med. Vet. Mycol.32:77-81.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/02681219680000261","volume":"34","year":"1996","unstructured":"Saraiva, E. C. O., A. Altemani, M. F. Franco, C. S. Unterkircher, and Z. P. Camargo. 1996. Paracoccidioides brasiliensis-gp43 used as paracoccidioidin. J. Vet. Med. Mycol.34:155-161.","journal-title":"J. Vet. Med. Mycol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/02681219780000811","volume":"35","year":"1997","unstructured":"Souza, M. C., J. L. Gesztesi, A. R. Souza, J. Z. Moraes, J. D. Lopes, and Z. P. Camargo. 1997. Differences in reactivity of paracoccidioidomycosis sera with gp43 isoforms. J. Med. Vet. Mycol.35:13-18.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0147-5975(89)90044-3","volume":"13","year":"1989","unstructured":"Taba, M. R. M., J. F. Silveira, L. R. Travassos, and S. Schenkman. 1989. Expression in Escherichia coli of a gene coding for epitopes of a diagnostic antigen of Paracoccidioides brasiliensis. Exp. Mycol.13:223-230.","journal-title":"Exp. Mycol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/02681219380000171","volume":"31","year":"1993","unstructured":"Taborda, C. P., and Z. P. Camargo. 1993. Diagnosis of paracoccidioidomycosis by passive hemagglutination assay for antibody using a purified and specific antigen gp43. J. Med. Vet. Mycol.31:155-160.","journal-title":"J. Med. Vet. Mycol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.786-793.1998"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.4.1465-1469.1994"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.9.6.1200-1204.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.9.6.1200-1204.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T16:32:51Z","timestamp":1627576371000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.9.6.1200-1204.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1128\/CDLI.9.6.1200-1204.2002"],"URL":"https:\/\/doi.org\/10.1128\/cdli.9.6.1200-1204.2002","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11]]},"assertion":[{"value":"2002-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}