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The results were compared with those from the indirect immunofluorescent antibody test (IFAT). A total of 1,822 canine sera were tested, including sera from dogs with visceral leishmaniasis, transmissible venereal tumors, ehrlichiosis, rickettsiosis, or Chagas' disease and sera from healthy dogs. The antigen was also tested with 227 samples of human sera, including sera from patients with visceral, cutaneous, or diffuse cutaneous leishmaniasis and from noninfected individuals, as well as sera from patients with Chagas' disease, toxoplasmosis, rickettsiosis, hepatitis B, schistosomiasis, ascaridiasis, malaria, rheumatoid factor, leprosy and rheumatoid factor, tuberculosis, or leprosy. All dogs and all human patients had a clinical and\/or serological and\/or parasitological diagnosis. For detecting antibodies in sera from dogs with leishmaniasis, the antigen showed a sensitivity of 98%, specificity of 95%, and concordance of 93% and when used for detecting antibodies in human sera presented a sensitivity of 92%, specificity of 100%, and concordance of 92%. Comparison between ELISA and IFAT demonstrated that ELISA using the LMS antigen yielded more reliable results than IFAT. The LMS antigen displayed no cross-reactivity with sera from patients or dogs that had any of the other diseases tested.<\/jats:p>","DOI":"10.1128\/cdli.9.6.1361-1366.2002","type":"journal-article","created":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T19:04:24Z","timestamp":1036177464000},"page":"1361-1366","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["<i>Leishmania major<\/i>-Like Antigen for Specific and Sensitive Serodiagnosis of Human and Canine Visceral Leishmaniasis"],"prefix":"10.1128","volume":"9","author":[{"given":"Rosa\u0302ngela","family":"Barbosa-de-Deus","sequence":"first","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Lui\u0301z","family":"dos Mares-Guia","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriane Zacarias","family":"Nunes","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ka\u0301tia Morais","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Gonc\u0327alves","family":"Junqueira","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson","family":"Mayrink","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odair","family":"Genaro","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Alberto Pereira","family":"Tavares","sequence":"additional","affiliation":[{"name":"Departamento de Farma\u0301cia, UFOP"},{"name":"Departamento de Bioqui\u0301mica-Imunologia"},{"name":"Departamento de Parasitologia, Instituto de Cie\u0302ncias Biolo\u0301gicas"},{"name":"Departamento de Alimentos, Faculdade de Farma\u0301cia, Universidade Federal de Minas Gerais"},{"name":"Fundac\u0327a\u0303o Ezequiel Dias, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0035-9203(76)90164-4","volume":"70","year":"1976","unstructured":"Ambroise-Thomas, P. 1976. 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