{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:48:08Z","timestamp":1761630488378},"reference-count":9,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We have evaluated a neutralizing antibody assay which uses human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 pseudovirions carrying a secretory alkaline phosphatase reporter gene and which can potentially measure functionally relevant HPV type-specific neutralizing antibodies. The reproducibility of the assay was excellent; for HPV-16, the intra- and interassay kappa values were 0.95 and 0.90, respectively; and for HPV-18, the corresponding values were 0.90 and 0.90. This assay was used to describe the kinetics of the neutralizing antibody response in a cohort of 42 young women who were recruited soon after first intercourse and who first tested positive for HPV-16 DNA or HPV-18 DNA, or both, during follow-up. Most women seroconverted following the first detection of type-specific HPV DNA and remained seropositive until the end of follow-up. Our findings are broadly consistent with those of two other cohort studies which have measured the serological response following an incident infection by using the technically simpler virus-like-particle-based enzyme-linked immunosorbent assay.<\/jats:p>","DOI":"10.1128\/cvi.00013-08","type":"journal-article","created":{"date-parts":[[2008,7,17]],"date-time":"2008-07-17T01:39:58Z","timestamp":1216258798000},"page":"1387-1390","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Measurement of the Humoral Immune Response following an Incident Human Papillomavirus Type 16 or 18 Infection in Young Women by a Pseudovirion-Based Neutralizing Antibody Assay"],"prefix":"10.1128","volume":"15","author":[{"given":"Jane","family":"Steele","sequence":"first","affiliation":[{"name":"CRUK Institute for Cancer Studies"},{"name":"CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart","family":"Collins","sequence":"additional","affiliation":[{"name":"CRUK Institute for Cancer Studies"},{"name":"CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaisheng","family":"Wen","sequence":"additional","affiliation":[{"name":"CRUK Institute for Cancer Studies"},{"name":"CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon","family":"Ryan","sequence":"additional","affiliation":[{"name":"CRUK Institute for Cancer Studies"},{"name":"CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christothea","family":"Constandinou-Williams","sequence":"additional","affiliation":[{"name":"CRUK Institute for Cancer Studies"},{"name":"CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciaran B. J.","family":"Woodman","sequence":"additional","affiliation":[{"name":"CRUK Institute for Cancer Studies"},{"name":"CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"445","volume":"119","year":"2005","unstructured":"Buck, C. B., D. V. Pastrana, D. R. Lowy, and J. T. Schiller. 2005. Generation of HPV pseudovirions using transfection and their use in neutralization assays. Methods Mol. Med.119:445-462.","journal-title":"Methods Mol. Med."},{"key":"e_1_3_2_3_2","first-page":"911","volume":"181","year":"2000","unstructured":"Carter, J. J., L. A. Koutsky, J. P. Hughes, S. K. Lee, J. Kuypers, N. Kiviat, et al. 2000. Comparison of human papillomavirus types 16, 18, and 6 capsid antibody responses following incident infection. J. Infect. Dis.181:911-919.","journal-title":"J. Infect. 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Genital human papillomavirus infection: incidence and risk factors in a cohort of female university students. Am. J. Epidemiol.157:218-226.","journal-title":"Am. J. Epidemiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1016\/S0140-6736(00)04956-4","volume":"357","year":"2001","unstructured":"Woodman, C. B. J., S. Collins, H. Winter, A. Bailey, J. Ellis, P. Prior, M. Yates, T. P. Rollason, and L. S. Young. 2001. Natural history of cervical human papillomavirus infection in young women: a longitudinal study. 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