{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:34:10Z","timestamp":1791120850525,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Western blot analysis of<jats:named-content content-type=\"genus-species\">Orientia tsutsugamushi<\/jats:named-content>whole-cell lysates with scrub typhus patient sera has identified at least five protein antigens of<jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>with molecular sizes of 22 kDa, 47 kDa, 56 kDa, 58 kDa, and 110 kDa. In this study, sera from serial bleedings of 108 patients were used to study the kinetics and the magnitude of specific antibody responses against the 47-kDa and 56-kDa antigens. Recombinant protein of the conserved 47-kDa antigen (r47b) or a mixture of truncated 56-kDa antigen (r56s) from three prototype strains was used as the antigen in an enzyme-linked immunosorbent assay (ELISA). Our results showed that 76% and 93% of these patients had elevated IgM and IgG against r47b, respectively, and 98% and 100% had elevated IgM and IgG against r56s, respectively. The kinetics of antibody responses against r47b and r56s can be grouped into three patterns. In the first type of response, IgM and IgG against r47b and r56s appeared about the same time. The IgM and IgG titers against r56s were much higher than those against r47b. In the second type of response, induction of IgM appeared to be similar to that in the first type. The major difference to the first type is that the IgG titers against r47b were induced at least 1 week later than those against the r56s. The third type showed strong IgG responses against both r47b and r56s, and low or no IgM responses indicated a secondary infection. This is the first systematic investigation of antibody response kinetics against the conserved 47-kDa antigen versus the variable 56-kDa antigen in scrub typhus patients.<\/jats:p>","DOI":"10.1128\/cvi.00017-11","type":"journal-article","created":{"date-parts":[[2011,4,21]],"date-time":"2011-04-21T00:16:16Z","timestamp":1303344976000},"page":"1021-1027","source":"Crossref","is-referenced-by-count":30,"title":["Kinetics and Magnitude of Antibody Responses against the Conserved 47-Kilodalton Antigen and the Variable 56-Kilodalton Antigen in Scrub Typhus Patients"],"prefix":"10.1128","volume":"18","author":[{"given":"Hua-Wei","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Huber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elissa","family":"Mutumanje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Chung","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Mei","family":"Ching","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2011,4,20]]},"reference":[{"key":"key20180919073516_B1","doi-asserted-by":"crossref","first-page":"532","DOI":"10.4269\/ajtmh.1982.31.532","article-title":"Humoral and cellular responses in scrub typhus patients reflecting primary infection and reinfection with Rickettsia tsutsugamushi","volume":"31","author":"Bourgeois","year":"1982","journal-title":"Am. 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Immun."},{"key":"key20180919073516_B11","first-page":"169","article-title":"Rickettsiosis in Japan","volume":"58","author":"Kawamura","year":"1988","journal-title":"Jpn. J. Exp. Med."},{"key":"key20180919073516_B12","doi-asserted-by":"crossref","first-page":"S145","DOI":"10.1086\/339908","article-title":"The past and present threat of rickettsial diseases to military medicine and international public health","volume":"34","author":"Kelly","year":"2002","journal-title":"Clin. Infect. Dis."},{"key":"key20180919073516_B13","doi-asserted-by":"crossref","first-page":"S203","DOI":"10.1086\/596576","article-title":"Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientia tsutsugamushi","volume":"48","author":"Kelly","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"key20180919073516_B14","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1128\/JCM.31.3.598-605.1993","article-title":"High-level expression of a 56-kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays","volume":"31","author":"Kim","year":"1993","journal-title":"J. Clin. Microbiol."},{"key":"key20180919073516_B15","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1128\/JCM.31.8.2057-2060.1993","article-title":"Rapid diagnosis of scrub typhus by a passive hemagglutination assay using recombinant 56-kilodalton polypeptides","volume":"31","author":"Kim","year":"1993","journal-title":"J. Clin. 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