{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:29:30Z","timestamp":1792373370975,"version":"4.4.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of\n                    <jats:named-content content-type=\"genus-species\">Leishmania infantum<\/jats:named-content>\n                    infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of\n                    <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n                    infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate\n                    <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n                    -infected animals from\n                    <jats:named-content content-type=\"genus-species\">Trypanosoma cruzi<\/jats:named-content>\n                    -infected animals and healthy animals. Soluble\n                    <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n                    antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate\n                    <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n                    -infected dogs from healthy and\n                    <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n                    -infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from\n                    <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n                    -infected dogs were used. The study's findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of\n                    <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n                    infection in dogs.\n                  <\/jats:p>","DOI":"10.1128\/cvi.00023-13","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T03:03:57Z","timestamp":1365044637000},"page":"835-841","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Sensitive and Specific Serodiagnosis of Leishmania infantum Infection in Dogs by Using Peptides Selected from Hypothetical Proteins Identified by an Immunoproteomic Approach"],"prefix":"10.1128","volume":"20","author":[{"given":"Miguel A.","family":"Ch\u00e1vez-Fumagalli","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian T.","family":"Martins","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam C. 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